Claim · model-written summary
The duplex PCR is a valuable diagnostic tool for monitoring BRR pathogens in cotton in Australia.
- Taxon
- cotton
Gossypium hirsutume6 - Outcome
- valuable diagnostic tool for detecting and monitoring the prevalence and distribution of BRR pathogense6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“This duplex PCR provides valuable diagnostic tool for detecting and monitoring the prevalence and distribution of BRR pathogens in cotton in Australia”
Title
Chi P. T. Nguyen; Andrew Chen; Duy P. Le Australasian Plant Pathology 2026. doi:10.1007/s13313-026-01096-z
Other claims from this paper (5)
- Method / resource The duplex PCR assay identifies B. rouxiae and B. basicola simultaneously without sequencing.
- Method / resource A new primer specifically amplifies a 159 bp amplicon of B. basicola.
- Method / resource The duplex PCR assay detects the two pathogens simultaneously at a limit of about 0.05 ng/µL.
- Method / resource The duplex PCR is sensitive enough to detect B. rouxiae from crude extracts prepared with a 5-min microwave-based DNA extraction.
- Method / resource Nested PCR combined with the duplex PCR successfully detects the BRR pathogen in 15–30 mg of diseased cotton tissue.