Claim · model-written summary
mRNA from irradiated peanut tissue hybridized with control DNA at about 20% of the level obtained with mRNA from control tissue.
- Taxon
- peanut
Arachis hypogaeae0 - Treatment
- irradiated tissuee7
- Outcome
- hybridized with control DNAe7
- Direction
- decreaseinferrede7
- Value
- about 20%e7
- Comparator
- that from control tissuee7
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of peanut seed to 500 kr of x-rays followed by 6 weeks of storage before planting resulted in a severe reduction of 14C-amino acid incorporation by the ribosomal material”
e7“mRNA, purified by methylated albumin Kieselguhr column chromatography from irradiated tissue, hybridized with control DNA about 20% as well as that from control tissue”
Title
R B van Huystee; W Jachymczyk; C F Tester; et al. Journal of Biological Chemistry 1968. doi:10.1016/s0021-9258(18)93477-0
Other claims from this paper (8)
- Intervention effect X-irradiation of peanut seed followed by storage severely reduced 14C-amino acid incorporation by the ribosomal material.
- Intervention effect Ribosomes from irradiated peanut tissue were as effective as those from control tissue in amino acid incorporation directed by exogenous mRNA and polyuridylic acid.
- Intervention effect Polyribosomes were nearly absent from ribosomal material of irradiated peanut tissue.
- Intervention effect Synthesis of RNA and protein in vivo was much less in irradiated peanut cotyledons than in control cotyledons.
- Intervention effect The 105,000 x g supernatant from irradiated peanut tissue was superior to the control supernatant when control ribosomes were used.
- Intervention effect The 105,000 x g supernatant from irradiated peanut tissue gave the same activity as control with ribosomes from irradiated tissue.
- Intervention effect Supernatant from irradiated peanut tissue contained only one-third as much ribonuclease activity as the control.
- Mechanism High RNase activity in the control supernatant may degrade the template and ribosomal RNAs, thereby reducing amino acid incorporation.