Claim · model-written summary
ARF16-2 is active in parenchyma and root stele of mature nodules.
- Taxon
- Soybean
Glycine maxt0 - Organ / stage
- parenchyma and root stele of mature nodulese0
- Gene
- ARF16-2e0
- Outcome
- activee0
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ARF16-2 promoter:GUS or tdT fusions show that this gene is active in the parenchyma and root stele of mature nodules.”
t0“Soybean”
Title
Regulation of ARF16-2 by microRNA160 During Soybean Root Nodule Development
Spencer Schreier Open PRAIRIE (South Dakota State University) 2017
Other claims from this paper (8)
- Observation ARF16-2 is transcriptionally active in primary root tips, lateral root primordium and emerging nodules.
- Observation ARF16-2 translational fusion signal was reduced in mature nodule tissues.
- Mechanism The reduced translational signal suggests post-transcriptional ARF16-2 regulation, likely by miR160 during nodule maturation.
- Observation ARF16-2 appears to bind the conserved AuxRE TGTCTC motif with lower affinity than activator ARFs.
- Mechanism The binding result suggests alternative binding sites or functionality for ARF16-2.
- Observation ARF16-2 appears to function as a monomer whereas activator ARFs appear to act as dimers.
- Mechanism The authors hypothesize that ARF16-2 heterodimerization with activator ARFs reduces target-gene transactivation.
- Method / resource A protein-DNA binding assay using a simple UV trans-illuminator was developed as part of the research.