Claim · model-written summary
Epicotyl elongation and true leaf formation were infrequent.
- Taxon
- American chestnut
Castanea dentatat0 - Outcome
- epicotyl elongation and formation of true leaves was infrequente0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“epicotyl elongation and formation of true leaves was infrequent”
t0“American chestnut”
Title
Plantlet regeneration from somatic embryos of American chestnut
Daniel T Carraway; Scott A Merkle Canadian Journal of Forest Research 1997. doi:10.1139/x97-123
Other claims from this paper (13)
- Observation Embryogenic response across treatments, genotypes and explant types was 0.9%.
- Observation Ovules and smaller embryos and cotyledons gave the most efficient embryogenic culture induction.
- Intervention effect Only cultures continuously maintained on 2,4-D sustained somatic embryo production.
- Intervention effect Benzyladenine reduced embryogenic culture induction about threefold.
- Intervention effect Liquid woody plant medium with 3 mg/L 2,4-D and 0.25 mg/L BA gave the fastest culture growth.
- Intervention effect Activated charcoal enhanced somatic embryo yield and growth.
- Intervention effect Sucrose promoted more somatic embryos than fructose.
- Intervention effect Fructose promoted a higher rate of single somatic embryo development than sucrose.
- Intervention effect Chilling at 4∞C enhanced root development.
- Observation Plantlets survived more than 60 days after transplantation.
- Observation Plantlets failed to continue growing after transplantation.
- Mechanism Fungus gnat larvae damage and high peat content were believed to contribute to failure of ex vitro survival.
- Recommendation Improving embryo maturation and conversion was recommended to increase system efficiency.