Claim · model-written summary
phot1 R472H is poorly functional under low-light conditions.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- phot1 R472He3
- Treatment
- low-light conditionse3
- Outcome
- poorly functionale3
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis phototropin 1”
e3“phot1 R472H is poorly functional under low-light conditions”
Title
Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1
Jan Petersen; Shin-ichiro Inoue; Sharon M. Kelly; et al. Journal of Biological Chemistry 2017. doi:10.1074/jbc.m117.799643
Other claims from this paper (6)
- Observation The histidine substitution of Arg-472 constitutively activates phot1 kinase activity in vitro without affecting LOV2 photochemistry.
- Observation phot1 R472H is autophosphorylated in darkness in vivo but cannot initiate phot1 signaling without light.
- Observation At higher light intensities phot1 R472H restores phototropism, chloroplast accumulation and stomatal opening.
- Observation At higher light intensities phot1 R472H restores leaf positioning and expansion.
- Mechanism Arabidopsis appears to adapt to the elevated phosphorylation of phot1 R472H in part by reducing its stability.
- Mechanism High-light activity of the mutant may be attributed to additional increases in LOV2-mediated photoreceptor autophosphorylation.