Claim · model-written summary
Propidium iodide staining validated the disruption of cell membrane integrity.
- Outcome
- disruption of cell membrane integritye0
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Verbatim
e0“Assessment with propidium iodide staining validated the disruption of cell membrane integrity”
Title
Bin Wang; Caixia Hou; Wei Chen; et al. Biological Control 2025. doi:10.1016/j.biocontrol.2025.105900
Other claims from this paper (14)
- Intervention effect YBG8 fermentation filtrate had a good control effect on root rot of Lycium barbarum caused by Fusarium solani.
- Intervention effect YBG8 fermentation filtrate treatment significantly impeded the radial growth of F. solani.
- Intervention effect YBG8 fermentation filtrate treatment altered the spore surface morphology of F. solani.
- Intervention effect YBG8 fermentation filtrate treatment triggered an upsurge in alkaline phosphatase activity in F. solani.
- Intervention effect YBG8 fermentation filtrate treatment resulted in heightened levels of malondialdehyde (MDA), with the target organism unstated in the sentence (context: F. solani).
- Intervention effect YBG8 fermentation filtrate treatment resulted in increased electrical conductivity.
- Intervention effect YBG8 fermentation filtrate treatment resulted in notable leakage of intracellular proteins and nucleic acids.
- Observation LC-MS analysis of the Alternaria alternata YBG8 fermentation filtrate revealed 252 metabolites, including coumarins, carboxylic acid derivatives and purine nucleosides.
- Observation Fifteen constituents of the filtrate exhibited concentrations exceeding 10 ug/mL.
- Observation Alternariol methyl ether was the predominant polyketide compound in the filtrate, at 130.086 ug/mL.
- Observation Altenusin, a diphenyl ether derivative, was detected in the filtrate at 26.664 ug/mL.
- Observation Altenuene, a cell wall synthesis inhibitor, was detected in the filtrate at 18.291 ug/mL.
- Mechanism The YBG8 fermentation filtrate is suggested to inhibit the growth of F. solani through multiple pathways such as cell wall disruption, oxidative stress induction and membrane function interference.
- Mechanism Alternariol methyl ether, altenusin and altenuene are presumed to be the primary antimicrobial agents in the fermentation filtrate.