Claim · model-written summary
Cell wall thionins in etiolated leaf material could be immunogold labeled specifically by the precursor antiserum.
- Taxon
- Barley
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Hordeum vulgaret0 - Organ / stage
- etiolated leaf materiale6
- Gene
- cell wall thioninse6
- Outcome
- immunogold labelede6
Not reportedMaterialCompoundInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
t0“Intracellular Thionins of Barley”
e6“In ultrathin sections of embedded etiolated leaf material, cell wall thionins could be immunogold labeled specifically by an antiserum raised against a fusion protein of Escherichia coli beta-galactosidase and the 15,000 Mr precursor polypeptide of thionins”
Title
Intracellular Thionins of Barley
U Reimann-Philipp; G Schrader; E Martinoia; et al. Journal of Biological Chemistry 1989. doi:10.1016/s0021-9258(18)81890-7
Other claims from this paper (8)
- Observation A second subfraction of thionins was detected mainly in the vacuole of barley leaf cells.
- Observation Thionins are toxic to phytopathogenic fungi.
- Observation Thionins are toxic to plant protoplasts.
- Intervention effect Light down-regulates thionin synthesis in etiolated barley seedlings.
- Observation Both thionin subfractions share similar amino acid sequences.
- Observation The two thionin subfractions are clearly distinguished by their subcellular distribution.
- Observation The precursor antiserum did not react with intracellular thionins.
- Observation Intracellular thionins were recognized specifically by an antiserum raised against soluble leaf thionins.