Claim · model-written summary
Acc65I/MseI methylation levels did not differ significantly among the three groups of regenerants.
- Taxon
- Gentiana cruciata
Gentiana cruciatat0 - Material
- three groups of regenerantse5
- Outcome
- Acc65I/MseI methylation levelse5
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“embryogenic capacity of Gentiana cruciata (L.) suspension culture”
e5“The Acc65I/MseI methylation levels for the three groups of regenerants were not significantly different”
Title
Anna Mikuła; Karolina Tomiczak; Jan J. Rybczyński Plant Cell Reports 2010. doi:10.1007/s00299-010-0970-1
Other claims from this paper (9)
- Observation Cryopreserved embryogenic suspension culture of Gentiana cruciata kept more than 80% viability after short- and long-term cryostorage.
- Intervention effect Osmotic dehydration increased somatic embryogenesis efficiency ten times in the proembryogenic mass.
- Intervention effect Air desiccation and cryostorage cryotreatments did not significantly alter somatic embryo production.
- Observation Two new AFLP unique DNA fragments were found in regenerants from cryopreserved tissue compared with non-cryotreated controls.
- Observation Regenerants from cryopreserved tissue showed no differences at the methylation level.
- Observation Cluster analysis identified a number of sub-clusters among the regenerants.
- Observation Only one sub-cluster comprised almost all regenerants from non-cryotreated and short-term cryostored tissue.
- Observation Plantlets from long-term cryostored tissue were grouped into separate clusters.
- Association The observed AFLP alterations were probably not associated with cryopreservation but related to in vitro culture.