Claim · model-written summary
Chelerythrine (10 nM) inhibits release of Plasmopara viticola zoospores from sporangia in a dose-dependent manner.
- Treatment
- chelerythrine (10 nM)e7
- Interactor
- P. viticola
Plasmopara viticolae7pathogen - Outcome
- release of zoospores from the P. viticola sporangiae7
- Direction
- inhibitede7
- Value
- 10 nMe7
- Qualifier
- in a dose-dependent mannere7
VitisNot reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“grapevine downy mildew pathogen Plasmopara viticola”
e7“Interestingly, both staurosporine (5 nM) and chelerythrine (10 nM) also inhibited the release of zoospores from the P. viticola sporangia in a dose-dependent manner”
Title
Md. Tofazzal Islam; Andreas von Tiedemann; Hartmut Laatsch Molecular Plant-Microbe Interactions 2011. doi:10.1094/mpmi-12-10-0280
Other claims from this paper (9)
- Intervention effect Extracts of a marine Streptomyces sp. rapidly impaired zoospore motility of Plasmopara viticola at 0.1 μg/ml.
- Intervention effect Staurosporine (2 nM) makes zoospores move very slowly or spin in tight circles instead of swimming straight.
- Intervention effect K-252a, K-252b and K-252c impaired zoospore motility in a similar manner to staurosporine, at varying doses.
- Intervention effect Chelerythrine was the most potent inhibitor of zoospore motility among 22 known kinase inhibitors, active from 5 nM.
- Intervention effect Inhibitors of kinase pathways other than PKC did not practically impair zoospore motility.
- Intervention effect Staurosporine (5 nM) inhibits release of Plasmopara viticola zoospores from sporangia in a dose-dependent manner.
- Intervention effect Staurosporine at 2 μM completely suppressed downy mildew disease in grapevine leaves.
- Mechanism PKC is likely a key signalling mediator of zoosporogenesis and flagellar motility maintenance in peronosporomycete zoospores.
- Observation Staurosporine was identified as the active principle in the Streptomyces extracts.