Claim · model-written summary
Twelve walnut-specific endogenous Pol III promoters significantly enhanced CRISPR/Cas9 editing efficiency when targeting JrPDS.
- Taxon
- Walnut (Juglans regia L.)
Juglans regiae0 - Treatment
- walnut-specific endogenous Pol III promoterse4
- Gene
- JrPDSe4
- Outcome
- CRISPR/Cas9-editing efficiencye4
- Direction
- significantly enhancee4
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Walnut (Juglans regia L.)”
e4“12 walnut-specific endogenous Pol III promoters (JrU3 and JrU6) were cloned and validated for their ability to significantly enhance CRISPR/Cas9-editing efficiency by targeting the walnut phytoene desaturase gene (JrPDS)”
Title
Li Song; Pu Zhang; Ruixue Gao; et al. Horticulture Research 2025. doi:10.1093/hr/uhaf187
Other claims from this paper (7)
- Observation Walnut cultivar HT-14 had the highest embryogenic induction (53.33%) among the cultivars screened.
- Observation Walnut cultivar HT-14 had the highest regeneration efficiency (85.33%) among the cultivars screened.
- Observation The 35S:RUBY construct was effectively expressed in somatic embryos of the HT-14 genotype.
- Intervention effect Endogenous promoters significantly increased walnut mutation efficiencies compared with commonly used exogenous promoters.
- Observation The JrU3-chr3 promoter achieved an editing efficiency of 58.82% in walnut.
- Intervention effect Endogenous promoters promoted higher frequencies of homozygous and biallelic mutations than exogenous promoters.
- Intervention effect Endogenous promoters promoted greater mutation diversity in walnut.