Claim · model-written summary
The velocity versus PEP concentration response curve was hyperbolic at pH 8.0.
- Taxon
- Crop Speciest0
- Outcome
- response curves of velocity versus substrate (PEP) concentratione7
- Qualifier
- hyperbolic at pH 8.0e7
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Verbatim
t0“Different Crop Species”
e7“the response curves of velocity versus substrate (PEP) concentration was hyperbolic at pH 8.0 and sigmoid at pH 7.0”
Title
D. J. Liu; C. S. Wang; Ma Wei 中華農業研究 1987. doi:10.29951/jarc.198709.0006
Other claims from this paper (11)
- Intervention effect Crops grew better with nitrate N than with ammonium N.
- Intervention effect Shoots of rice and wheat gained more fresh weight with ammonium N.
- Intervention effect Ammonium N increased PEP carboxylase activity of various crops by 8-61% in the leaf blade.
- Intervention effect Ammonium N increased PEP carboxylase activity in the root by as much as 4.5 folds.
- Intervention effect Ammonium-N at a very low concentration significantly enhanced root PEP carboxylase activity.
- Observation Optimal pH for rice root PEP carboxylase was between 8.0 and 8.5 in Tris-HCl or Hepes-KOH buffer.
- Observation PEP carboxylase from the roots of Tainung 67 rice showed no decrease in enzyme activity up to 7 hours after extraction.
- Observation The velocity versus PEP concentration response curve was sigmoid at pH 7.0.
- Observation Reciprocal plots of the data were linear, indicating Michaelis-Menten kinetics.
- Observation The Km for PEP of root PEP carboxylase was 0.16 mM at pH 8.0.
- Observation The ammonium-N responsive root PEP carboxylase was similar to leaf-blade PEP carboxylase of C3 plants in many aspects.