Claim · model-written summary
The first purified monomeric allantoinase protein had molecular mass 45 kDa.
- Taxon
- French bean
Phaseolus vulgarist0 - Outcome
- molecular massese0
- Value
- 45e0
- Qualifier
- monomerice0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both proteins with allantoinase activity are monomeric with molecular masses of 45 and 42 kDa.”
t0“French bean (Phaseolus vulgaris)”
Title
María José Raso; Manuel Pineda; Pedro Piedras Physiologia Plantarum 2007. doi:10.1111/j.1399-3054.2007.00969.x
Other claims from this paper (15)
- Observation Allantoinase activity occurred in all analysed tissues and was highest in developing fruits.
- Observation Chromatography yielded two peaks of allantoinase activity, whose proteins were independently purified.
- Method / resource Total allantoinase activity was purified 200-fold.
- Observation Allantoinase represented 0.5% of total soluble protein in developing fruits.
- Observation The second purified monomeric allantoinase protein had molecular mass 42 kDa.
- Observation The first purified protein had specific activity 560 units mg(-1).
- Observation The second purified protein had specific activity 295 units mg(-1).
- Observation The first protein had turnover number 25,200 min(-1).
- Observation The second protein had turnover number 12,100 min(-1).
- Observation Both proteins showed Michaelis-Menten kinetics for allantoin with Km about 60 mM and high optimal temperatures.
- Observation Both proteins were metalloenzymes.
- Intervention effect Compounds reacting with sulphydryl groups inhibited both proteins.
- Intervention effect Reducing agents did not affect the proteins.
- Observation All analysed tissues contained both allantoin-degrading activities, with different predominant forms in leaves and roots.
- Intervention effect Both proteins had the same activity and distribution pattern under nitrogen fixation and nitrate fertilization.