Claim · model-written summary
Adding 12 millimolar proline increased callus tolerance to 4 degrees C.
Zea maysNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline (12 millimolar) added to the culture media also increased the tolerance of callus to 4 degrees C.”
t0“Maize Callus”
Title
Proline Accumulation and Its Implication in Cold Tolerance of Regenerable Maize Callus
David R. Duncan; Jack M. Widholm PLANT PHYSIOLOGY 1987. doi:10.1104/pp.83.3.703
Other claims from this paper (7)
- Intervention effect Mannitol-induced water stress, cool temperature and ABA raised proline in maize callus to 2.1–2.5 times control.
- Intervention effect Combined mannitol and ABA raised callus proline to about 4.5 times control.
- Association Proline accumulation increased with mannitol level.
- Observation ABA above 1.0 micromolar was needed to induce proline accumulation comparable to mannitol.
- Intervention effect Mannitol and ABA levels maximizing proline inhibited callus growth.
- Intervention effect Mannitol and ABA levels maximizing proline increased callus cold tolerance.
- Intervention effect Mannitol and ABA permitted cold storage of maize callus for 90 days, more than double typical survival.