Claim · model-written summary
Antibody to mature NADP malic enzyme precipitated both the precursor and mature forms.
Zea maysNot reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the precursor and mature NADP malic enzyme are immunoprecipitated by antibody made against NADP malic enzyme purified from corn leaves”
t0“maize”
Title
Light-stimulated synthesis of NADP malic enzyme in leaves of maize.
P D Collins; D R Hague Journal of Biological Chemistry 1983. doi:10.1016/s0021-9258(18)32769-8
Other claims from this paper (10)
- Intervention effect Illumination for 80 h increased maize NADP malic enzyme activity 15-20-fold.
- Observation NADP malic enzyme protein increased alongside activity in illuminated maize.
- Observation Translatable mRNA for NADP malic enzyme increased alongside activity in illuminated maize.
- Observation Radiolabeled amino acids were incorporated into NADP malic enzyme during illumination.
- Mechanism The authors attribute greening-associated enzyme activity increases to de novo synthesis of NADP malic enzyme protein.
- Observation RNA from greening maize leaves directed in vitro synthesis of a protein 12,000 daltons larger than mature NADP malic enzyme.
- Observation NADP malic enzyme protein and precursor mRNA both increased during greening.
- Observation Peptide maps of precursor and mature NADP malic enzyme were very similar.
- Observation Mature and precursor NADP malic enzyme migrated as doublet bands.
- Observation Peptide analyses found all bands structurally related.