Abstract
Illumination of etiolated maize plants for 80 h brings about a 15-20-fold increase in activity of NADP malic enzyme (EC 1.1.1.40). Increases in NADP malic enzyme protein and in the level of translatable mRNA for this protein occur simultaneously with the activity increase. Radiolabeled amino acids are also incorporated into NADP malic enzyme during this time. These results are consistent with the conclusion that an increase in NADP malic enzyme activity during greening results from de novo synthesis of NADP malic enzyme protein. Polyadenylated RNA extracted from greening maize leaves directs the synthesis in vitro of a protein 12,000 daltons larger than NADP malic enzyme purified from corn leaves. This protein is a precursor of NADP malic enzyme because 1) both the precursor and mature NADP malic enzyme are immunoprecipitated by antibody made against NADP malic enzyme purified from corn leaves, 2) both NADP malic enzyme protein and the level of mRNA for the precursor increase during greening, and 3) peptide maps of the precursor and of mature NADP malic enzyme are very similar. Mature NADP malic enzyme and its precursor (synthesized in vitro) both migrate on sodium dodecyl sulfate-polyacrylamide gradient gels as doublet bands. Peptide analyses show all bands to be structurally related.
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Claims 11
Claim · model-written summary
Illumination for 80 h increased maize NADP malic enzyme activity 15-20-fold.
- Treatment
- Illumination of etiolated maize plants for 80 he0
- Gene
- NADP malic enzymee0
- Outcome
- activity of NADP malic enzymee0
- Direction
- increasee0
- Value
- 15-20-folde0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Illumination of etiolated maize plants for 80 h brings about a 15-20-fold increase in activity of NADP malic enzyme (EC 1.1.1.40).”
Claim · model-written summary
NADP malic enzyme protein increased alongside activity in illuminated maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increases in NADP malic enzyme protein and in the level of translatable mRNA for this protein occur simultaneously with the activity increase.”
t0“maize”
Claim · model-written summary
Translatable mRNA for NADP malic enzyme increased alongside activity in illuminated maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increases in NADP malic enzyme protein and in the level of translatable mRNA for this protein occur simultaneously with the activity increase.”
t0“maize”
Claim · model-written summary
Radiolabeled amino acids were incorporated into NADP malic enzyme during illumination.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Radiolabeled amino acids are also incorporated into NADP malic enzyme during this time.”
t0“maize”
Claim · model-written summary
The authors attribute greening-associated enzyme activity increases to de novo synthesis of NADP malic enzyme protein.
- Gene
- NADP malic enzymee0
- Outcome
- NADP malic enzyme activitye0
- Qualifier
- de novo synthesis of NADP malic enzyme proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in NADP malic enzyme activity during greening results from de novo synthesis of NADP malic enzyme protein.”
t0“maize”
Claim · model-written summary
RNA from greening maize leaves directed in vitro synthesis of a protein 12,000 daltons larger than mature NADP malic enzyme.
- Organ / stage
- leavese0
- Gene
- NADP malic enzymee0
- Outcome
- a proteine0
- Direction
- largere0
- Value
- 12,000 daltonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polyadenylated RNA extracted from greening maize leaves directs the synthesis in vitro of a protein 12,000 daltons larger than NADP malic enzyme purified from corn leaves.”
Claim · model-written summary
Antibody to mature NADP malic enzyme precipitated both the precursor and mature forms.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the precursor and mature NADP malic enzyme are immunoprecipitated by antibody made against NADP malic enzyme purified from corn leaves”
t0“maize”
Claim · model-written summary
NADP malic enzyme protein and precursor mRNA both increased during greening.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both NADP malic enzyme protein and the level of mRNA for the precursor increase during greening”
t0“maize”
Claim · model-written summary
Peptide maps of precursor and mature NADP malic enzyme were very similar.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peptide maps of the precursor and of mature NADP malic enzyme are very similar.”
t0“maize”
Claim · model-written summary
Mature and precursor NADP malic enzyme migrated as doublet bands.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature NADP malic enzyme and its precursor (synthesized in vitro) both migrate on sodium dodecyl sulfate-polyacrylamide gradient gels as doublet bands.”
t0“maize”
Claim · model-written summary
Peptide analyses found all bands structurally related.
- Outcome
- structurally relatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Peptide analyses show all bands to be structurally related.”
t0“maize”
The same questions in other papers
light · physiology: enzyme activity · Zea mays (maize)Positive and negative results
11 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- no effect
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.