Claim · model-written summary
A reproducible petiole-explant regeneration protocol was developed.
- Taxon
- Jatropha curcas
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Jatropha curcase0 - Outcome
- induction of adventitious shoot buds and plant regeneratione0
- Value
- high-frequencye0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“A high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from cotyledonary petiole explants of Jatropha curcas L. has been developed”
Title
Ying Liu; Jiannong Lu; Hongbo Zhu; et al. Biotechnology & Biotechnological Equipment 2016. doi:10.1080/13102818.2016.1199971
Other claims from this paper (11)
- Observation Direct TDZ culture produced poor-quality buds at low frequency.
- Intervention effect High-concentration TDZ pretreatment significantly increased regeneration frequency.
- Intervention effect High-concentration TDZ pretreatment improved shoot-bud quality.
- Observation Treatment with 20 mg/L TDZ for 20 min yielded 88.42% shoot-bud induction.
- Observation Treatment with 20 mg/L TDZ for 20 min yielded 12.67 shoot buds per explant.
- Association Explant orientation influenced regeneration.
- Association Petiole origin influenced regeneration.
- Intervention effect L-arginine addition aided shoot-bud elongation.
- Observation A concentration of 7.5 mg/L L-arginine gave the best elongation result.
- Observation Elongated shoots rooted in half-strength MS medium with 0.1 mg/L IBA.
- Observation Acclimatized plantlets were transplantable to soil and grew normally.