Abstract
A high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from cotyledonary petiole explants of Jatropha curcas L. has been developed. The cotyledonary petiole explants of J. curcas cultured directly on medium supplemented with thidiazuron (TDZ) induce regeneration of poor quality shoot buds that have a low regeneration frequency. However, treating the explants with high concentrations (10–60 mg/L) of TDZ solution for certain time periods (5–80 min) significantly increased the regeneration frequency and improved the quality of the regenerated shoot buds. The best shoot buds induction (88.42%) and number of shoot buds (12.67) per explant were observed when in vitro explants were treated with 20 mg/L TDZ solution for 20 min before being transferred on hormone-free medium after 30 days. Regeneration was also influenced by the orientation (horizontal or vertical) of the explants on the medium, and by the origin of the cotyledonary petioles (in vitro or in vivo) used for the preparation of explants. We performed subsequent experiments for elongation and rooting of the regenerated shoot buds. Addition of L-arginine to the medium was conducive to the elongation of the shoot buds. A concentration of 7.5 mg/L L-arginine yielded the best results. The elongated shoots could initiate roots to become intact plantlets in half-strength Murashige and Skoog medium containing 0.1 mg/L indole-3-butyric acid. After acclimatization, these plantlets could be transplanted to the soil and the growth was normal. Therefore, application of the methods described here helped to increase plant regeneration efficiency.
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Claims 12
Claim · model-written summary
A reproducible petiole-explant regeneration protocol was developed.
- Taxon
- Jatropha curcas
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Jatropha curcase0 - Outcome
- induction of adventitious shoot buds and plant regeneratione0
- Value
- high-frequencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“A high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from cotyledonary petiole explants of Jatropha curcas L. has been developed”
Claim · model-written summary
Direct TDZ culture produced poor-quality buds at low frequency.
- Taxon
- J. curcasJatropha curcase0
- Treatment
- medium supplemented with thidiazuron (TDZ)e0
- Outcome
- poor quality shoot buds that have a low regeneration frequencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cotyledonary petiole explants of J. curcas cultured directly on medium supplemented with thidiazuron (TDZ) induce regeneration of poor quality shoot buds that have a low regeneration frequency”
Claim · model-written summary
High-concentration TDZ pretreatment significantly increased regeneration frequency.
- Treatment
- high concentrations (10–60 mg/L) of TDZ solution for certain time periods (5–80 min)e0
- Outcome
- regeneration frequencye0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“treating the explants with high concentrations (10–60 mg/L) of TDZ solution for certain time periods (5–80 min) significantly increased the regeneration frequency and improved the quality of the regenerated shoot buds”
Claim · model-written summary
High-concentration TDZ pretreatment improved shoot-bud quality.
- Treatment
- high concentrations (10–60 mg/L) of TDZ solution for certain time periods (5–80 min)e0
- Outcome
- quality of the regenerated shoot budse0
- Direction
- improvede0
Not reportedTaxonComparator
Verbatim
e0“treating the explants with high concentrations (10–60 mg/L) of TDZ solution for certain time periods (5–80 min) significantly increased the regeneration frequency and improved the quality of the regenerated shoot buds”
Claim · model-written summary
Treatment with 20 mg/L TDZ for 20 min yielded 88.42% shoot-bud induction.
Not reportedTaxon
Verbatim
e0“The best shoot buds induction (88.42%) and number of shoot buds (12.67) per explant were observed when in vitro explants were treated with 20 mg/L TDZ solution for 20 min”
Claim · model-written summary
Treatment with 20 mg/L TDZ for 20 min yielded 12.67 shoot buds per explant.
Not reportedTaxon
Verbatim
e0“The best shoot buds induction (88.42%) and number of shoot buds (12.67) per explant were observed when in vitro explants were treated with 20 mg/L TDZ solution for 20 min”
Claim · model-written summary
Explant orientation influenced regeneration.
Not reportedTaxonDirection
Verbatim
e0“Regeneration was also influenced by the orientation (horizontal or vertical) of the explants on the medium”
Claim · model-written summary
Petiole origin influenced regeneration.
Not reportedTaxonDirection
Verbatim
e0“Regeneration was also influenced by the orientation (horizontal or vertical) of the explants on the medium, and by the origin of the cotyledonary petioles (in vitro or in vivo)”
Claim · model-written summary
L-arginine addition aided shoot-bud elongation.
- Treatment
- Addition of L-arginine to the mediume0
- Outcome
- elongation of the shoot budse0
- Direction
- conducivee0
Not reportedTaxonComparator
Verbatim
e0“Addition of L-arginine to the medium was conducive to the elongation of the shoot buds”
Claim · model-written summary
A concentration of 7.5 mg/L L-arginine gave the best elongation result.
Not reportedTaxon
Verbatim
e0“A concentration of 7.5 mg/L L-arginine yielded the best results”
Claim · model-written summary
Elongated shoots rooted in half-strength MS medium with 0.1 mg/L IBA.
- Organ / stage
- elongated shootse0
- Treatment
- half-strength Murashige and Skoog medium containing 0.1 mg/L indole-3-butyric acide0
- Outcome
- initiate roots to become intact plantletse0
Not reportedTaxon
Verbatim
e0“The elongated shoots could initiate roots to become intact plantlets in half-strength Murashige and Skoog medium containing 0.1 mg/L indole-3-butyric acid”
Claim · model-written summary
Acclimatized plantlets were transplantable to soil and grew normally.
Not reportedTaxon
Verbatim
e0“After acclimatization, these plantlets could be transplanted to the soil and the growth was normal”
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