Abstract
A high-frequency protocol for induction of adventitious buds and plant regeneration from cotyledon explant of Jatropha curcas is described. The cotyledon explants of J. curcas cultured directly onto medium containing Thidiazuron (TDZ) induced regeneration of only poor quality adventitious buds that had a low regeneration frequency, and subsequently the elongation of shoot-buds was difficult and unsatisfactory. However, treating the cotyledon explants with high concentrations (10-60 mg/L) of TDZ solution for short time periods (10-60 min) helped to improve the regeneration frequency and enhance the quality of the regenerated shoot-buds significantly. The best shoot-buds induction (87.45%) and number of shoot-buds (11.23) per explant were seen when in vitro explants were treated with 20 mg/L TDZ solution for 40 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium in 30 days. The elongated shoots initiated roots to become intact plantlets in rooting medium supplemented with 0.1 mg/L indole-3-butyric acid (IBA) effectively stimulated the initiation and growth of roots with the best rooting rate (44.28%). After acclimatization, these plantlets were transplanted to soil wherein normal growth was observed. Hence, an intact plantlet could usually often be gained at 60 to 70 days of culture by applying the culture method described in the present study. This protocol could be widely used for mass production of regenerative plants and the yield of transgenic plants through Agrobacterium-mediated transformation.
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Claims 7
Claim · model-written summary
Direct culture on TDZ medium produced poor quality buds at low frequency and unsatisfactory elongation.
- Taxon
- J. curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Organ / stage
- cotyledon explantse0
- Treatment
- medium containing Thidiazuron (TDZ)e0
- Outcome
- regeneration of only poor quality adventitious budse0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“cotyledon explants of J. curcas cultured directly onto medium containing Thidiazuron (TDZ) induced regeneration of only poor quality adventitious buds that had a low regeneration frequency, and subsequently the elongation of shoot-buds was difficult and unsatisfactory”
Claim · model-written summary
Brief treatment with 10–60 mg/L TDZ significantly improved bud regeneration frequency and quality.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- cotyledon explantse0
- Treatment
- high concentrations (10-60 mg/L) of TDZ solution for short time periods (10-60 min)e0
- Outcome
- regeneration frequency and enhance the quality of the regenerated shoot-budse0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treating the cotyledon explants with high concentrations (10-60 mg/L) of TDZ solution for short time periods (10-60 min) helped to improve the regeneration frequency and enhance the quality of the regenerated shoot-buds significantly”
t0“Jatropha curcas”
Claim · model-written summary
Twenty mg/L TDZ for 40 minutes gave the highest bud induction, 87.45%.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- 20 mg/L TDZ solution for 40 mine0
- Outcome
- shoot-buds inductione0
- Value
- 87.45%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best shoot-buds induction (87.45%) and number of shoot-buds (11.23) per explant were seen when in vitro explants were treated with 20 mg/L TDZ solution for 40 min”
t0“Jatropha curcas”
Claim · model-written summary
Twenty mg/L TDZ for 40 minutes produced 11.23 shoot buds per explant.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- 20 mg/L TDZ solution for 40 mine0
- Outcome
- number of shoot-budse0
- Value
- 11.23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best shoot-buds induction (87.45%) and number of shoot-buds (11.23) per explant were seen when in vitro explants were treated with 20 mg/L TDZ solution for 40 min”
t0“Jatropha curcas”
Claim · model-written summary
Rooting medium with 0.1 mg/L IBA gave a best rooting rate of 44.28%.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- 0.1 mg/L indole-3-butyric acid (IBA)e0
- Outcome
- rooting ratee0
- Value
- 44.28%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting medium supplemented with 0.1 mg/L indole-3-butyric acid (IBA) effectively stimulated the initiation and growth of roots with the best rooting rate (44.28%)”
t0“Jatropha curcas”
Claim · model-written summary
Transplanted plantlets grew normally in soil after acclimatization.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- normal growthe0
- Setting
- soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After acclimatization, these plantlets were transplanted to soil wherein normal growth was observed”
t0“Jatropha curcas”
Claim · model-written summary
The culture protocol can yield an intact plantlet in 60 to 70 days.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- intact plantlete0
- Value
- 60 to 70 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an intact plantlet could usually often be gained at 60 to 70 days of culture by applying the culture method described in the present study”
t0“Jatropha curcas”
The same questions in other papers
Thidiazuron · quality: general · Jatropha curcasResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · quality: general · Jatropha curcasResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.