Claim · model-written summary
SACMV BC1 mismatched constructs reduced viral replication in infected Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- SACMV BC1 mismatched IR constructse0
- Interactor
- SACMVe0pathogen
- Outcome
- viral replicatione0
- Direction
- reductione0
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SACMV BC1 mismatched IR constructs in the model test plant Nicotiana benthamiana resulted in a reduction in viral BC1 transcript levels, hence viral replication, upon SACMV infection.”
Title
Sarah H. Taylor; Johan Harmse; Patrick Arbuthnot; et al. BioTechniques 2012. doi:10.2144/0000113839
Other claims from this paper (5)
- Method / resource The Pdk intron did not stabilize SACMV silencing constructs.
- Method / resource A bisulfite mismatch method produced long SACMV inverted-repeat constructs.
- Intervention effect SACMV BC1 mismatched constructs reduced viral BC1 transcript levels in infected Nicotiana benthamiana.
- Intervention effect Mismatched SACMV AC1 constructs induced PTGS more efficiently than nonmismatched constructs in N. benthamiana callus.
- Recommendation The authors expect the new construct-generation method to apply to sequences for which constructs have been difficult to make.