Claim · model-written summary
M298S had decreased DMA in the 1.0-1.4 range.
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Hordeum vulgareNot reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“M53E, M298S, and T212W at subsites -2, +1/+2, and +4, respectively, and the double mutant Y105A/T212W had decreased DMA of 1.0-1.4”
t0“Barley”
Title
Birte Kramhøft; Kristian Sass Bak-Jensen; Haruhide Mori; et al. Biochemistry 2005. doi:10.1021/bi048100v
Other claims from this paper (19)
- Observation Wild-type barley AMY1 had a multiple attack degree of 1.9.
- Observation Y105A increased multiple attack degree to 3.3.
- Observation AMY1-SBD increased multiple attack degree to 3.0.
- Observation M53E had decreased DMA in the 1.0-1.4 range.
- Observation T212W had decreased DMA in the 1.0-1.4 range.
- Observation Y105A/T212W had decreased DMA in the 1.0-1.4 range.
- Observation C95A had DMA similar to wild type.
- Observation Maltoheptaose was the major initial oligosaccharide product.
- Observation Wild type and subsite mutants released G6 at 27-40% of the G7 rate.
- Observation Wild type and subsite mutants released G8 at 60-70% of the G7 rate.
- Observation Wild type and subsite mutants released G9 at 39-48% of the G7 rate.
- Observation Wild type and subsite mutants released G10 at 33-44% of the G7 rate.
- Observation AMY1-SBD produced G8 at a rate similar to G7.
- Observation AMY1-SBD produced G9 at 66% of the G7 rate.
- Observation AMY1-SBD produced G10 at 60% of the G7 rate.
- Observation G1 through G4 product rates varied substantially among variants.
- Observation G5 was consistently a minor product.
- Mechanism Long substrate translocation produced G6-G10 largely independently of subsite mutations.
- Mechanism Short substrate movements produced individually variable G1-G4 release rates.