A combination of small explants, cold hardening, sucrose preculture and PVS2 produced the highest shoot-tip regrowth after cryopreservation.

Claim · model-written summary

A combination of small explants, cold hardening, sucrose preculture and PVS2 produced the highest shoot-tip regrowth after cryopreservation.

Taxon

Citrus spp.Citrust0

Organ / stage

shoot tipe0

Treatment

cold hardened seedlings at 4 °C for three dayse0

Outcome

shoot tip regrowthe0

Direction

higheste0

Value

13.3 to 33.3%e0

Qualifier

0.3 to 0.7 mm explantse0

Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSetting

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim

t0

“Citrus spp.”

e0

“The highest shoot tip regrowth, ranging from 13.3 to 33.3%, was achieved when they were obtained from 0.3 to 0.7 mm explants excised from cold hardened seedlings at 4 °C for three days that were then precultured in a medium containing 0.25 M of sucrose and treated with PVS2 at 0 °C for 45 min.”