Claim · model-written summary
Dilution-induced phenylalanine ammonia-lyase production also occurred in cells that had lost their response to hormonal induction.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarist0 - Treatment
- dilutione1
- Outcome
- induction produced by dilutione1
- Direction
- also occurrede1
- Qualifier
- which had lost their ability to respond to the hormonal inductione1
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Phaseolus vulgaris”
e1“The induction produced by dilution also occurred in these cells which had lost their ability to respond to the hormonal induction”
Title
Michael Bevan; D. H. Northcote Journal of Cell Science 1979. doi:10.1242/jcs.39.1.339
Other claims from this paper (9)
- Intervention effect Repeated subculture in medium with only 2,4-dichlorophenoxyacetic acid progressively reduced phenylalanine ammonia-lyase activity and the number of induced xylem elements.
- Intervention effect Cells grown with coconut milk did not lose their ability to induce phenylalanine ammonia-lyase or xylem elements, relative to the medium with only 2,4-dichlorophenoxyacetic acid.
- Observation Clones from cells grown with coconut milk differed in the amount of phenylalanine ammonia-lyase produced after induction.
- Intervention effect Clones producing low amounts of activity did not grow faster in the medium lacking coconut milk.
- Mechanism No evidence was found that selective growth of non-inducible cells caused the loss of morphogenetic potential.
- Intervention effect Dilution at subculture stimulated phenylalanine ammonia-lyase activity in the cells.
- Intervention effect The activity increase after dilution occurred within 10 h, whereas the hormone-induced increase occurred after 120 h.
- Mechanism The mechanism producing the increase in phenylalanine ammonia-lyase activity was intact, but cells had lost their response to the induction-medium hormones.
- Mechanism The loss of inducibility was probably due to reversible changes in the hormonal requirements of the cells, not to a genetic change.