Abstract
SummaryThe availability and maintenance of embryogenic callus is a major limitation for large-scale application of somatic hybridization for citrus breeding. The suitability of cryopreserved callus as source of protoplasts was evaluated. Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen. Cryopreserved callus were fast thawed and used as source of material for protoplast isolation, protoplast fusion and plant regeneration, in comparison with control non-cryopreserved callus. No differences were found in protoplast yield, quality, growth and regeneration capacity between both callus types. Protoplasts isolated from cryopreserved callus were also successfully used in somatic fusion assays. Plants regenerated from protoplasts of the two sources had the same phenotypic characters and no differences were detected by microsatellite analysis. Availability of cryopreserved callus facilitates the development of breeding programmes based on somatic hybridization, avoiding the risks and high labour needs associated with standard maintenance by periodical subcultures.
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Claims 6
Claim · model-written summary
Protoplast yield, quality and growth did not differ between cryopreserved and non-cryopreserved sweet-orange callus.
- Taxon
- Sweet orange
Citrus sinensise1 - Treatment
- frozen by slow cooling and stored for two years in liquid nitrogene1
- Outcome
- protoplast yield, quality, growthe3
- Direction
- no_changeinferrede3
- Comparator
- non-cryopreserved calluse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen”
e2“in comparison with control non-cryopreserved callus”
e3“No differences were found in protoplast yield, quality, growth and regeneration capacity between both callus types”
Claim · model-written summary
Regeneration capacity did not differ between cryopreserved and non-cryopreserved sweet-orange callus.
- Taxon
- Sweet orangeCitrus sinensise1
- Treatment
- frozen by slow cooling and stored for two years in liquid nitrogene1
- Outcome
- regeneration capacitye3
- Direction
- no_changeinferrede3
- Comparator
- non-cryopreserved calluse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen”
e2“in comparison with control non-cryopreserved callus”
e3“No differences were found in protoplast yield, quality, growth and regeneration capacity between both callus types”
Claim · model-written summary
Protoplasts isolated from cryopreserved sweet-orange callus were successfully used in somatic fusion assays.
- Taxon
- Sweet orangeCitrus sinensise1
- Treatment
- cryopreserved calluse4
- Outcome
- somatic fusion assayse4
- Qualifier
- successfully usede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen”
e4“Protoplasts isolated from cryopreserved callus were also successfully used in somatic fusion assays”
Claim · model-written summary
Plants regenerated from protoplasts of both callus sources had the same phenotypic characters.
- Taxon
- Sweet orangeCitrus sinensise1
- Outcome
- phenotypic characterse5
- Qualifier
- the samee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen”
e5“Plants regenerated from protoplasts of the two sources had the same phenotypic characters and no differences were detected by microsatellite analysis”
Claim · model-written summary
No differences were detected by microsatellite analysis between plants regenerated from the two callus sources.
- Taxon
- Sweet orangeCitrus sinensise1
- Outcome
- microsatellite analysise5
- Qualifier
- no differences were detectede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Sweet orange callus were frozen by slow cooling and stored for two years in liquid nitrogen”
e5“Plants regenerated from protoplasts of the two sources had the same phenotypic characters and no differences were detected by microsatellite analysis”
Claim · model-written summary
Cryopreserved callus facilitates breeding programmes based on somatic hybridization by avoiding the risks and labour of periodical subcultures.
- Outcome
- development of breeding programmes based on somatic hybridizatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cryopreserved callus, a source of protoplasts for citrus improvement”
e6“Availability of cryopreserved callus facilitates the development of breeding programmes based on somatic hybridization”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.