Abstract
A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue. Inhibitor activity from fresh tissue decays with wounding. Inhibition activity was detected absorbed to ribsomal fraction and cytosol of potato tuber tissue by a partially reconstituted in vitro system from potato tuber and wheat germ. Adsorbed ribosomal fraction was more suitable of purification. This fraction was washed from ribosomes with 0.3M KCl, concentrated with ammonium sulfate precipitation and purified through sephadex G100 and sephadex G-75 columns chromatography. After 61 fold purification adsorbed protein fraction can inhibit germination of maize, wheat and sesame seeds, as well as {sup 3}H-leucine incorporation into protein by imbibed maize embryos. Inhibition activity was lost by temperature, alkali and protease-K hydrolysis. Preliminar analysis could not show presence of reductor sugars. Physiological role of this inhibitor in relation to rest and active tissue remains to be studied.
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Claims 5
Claim · model-written summary
Inhibitor activity of potato tuber tissue decays with wounding compared with fresh tissue.
Verbatim
e1“A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue.”
e2“Inhibitor activity from fresh tissue decays with wounding.”
Claim · model-written summary
The adsorbed protein fraction of potato tuber inhibited germination of maize, wheat and sesame seeds.
- Taxon
- potato tuberse1
- Material
- maize, wheat and sesame seedse3
- Treatment
- adsorbed protein fractione3
- Outcome
- germinatione3
- Direction
- inhibite3
Not reportedComparator
Verbatim
e1“A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue.”
e3“After 61 fold purification adsorbed protein fraction can inhibit germination of maize, wheat and sesame seeds, as well as {sup 3}H-leucine incorporation into protein by imbibed maize embryos.”
Claim · model-written summary
The adsorbed protein fraction of potato tuber inhibited incorporation of 3H-leucine into protein by imbibed maize embryos.
- Taxon
- potato tuberse1
- Organ / stage
- imbibed maize embryose3
- Treatment
- adsorbed protein fractione3
- Outcome
- incorporation into proteine3
- Direction
- inhibite3
Not reportedComparator
Verbatim
e1“A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue.”
e3“After 61 fold purification adsorbed protein fraction can inhibit germination of maize, wheat and sesame seeds, as well as {sup 3}H-leucine incorporation into protein by imbibed maize embryos.”
Claim · model-written summary
Inhibition activity of the potato tuber protein fraction was lost after temperature, alkali and protease-K hydrolysis.
- Taxon
- potato tuberse1
- Treatment
- temperature, alkali and protease-K hydrolysise4
- Outcome
- Inhibition activitye4
- Direction
- loste4
Not reportedComparator
Verbatim
e1“A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue.”
e4“Inhibition activity was lost by temperature, alkali and protease-K hydrolysis.”
Claim · model-written summary
Preliminary analysis of the potato tuber inhibitor showed no presence of reductor sugars.
Verbatim
e1“A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue.”
e5“Preliminar analysis could not show presence of reductor sugars.”