Abstract
Cassava (Manihot esculenta Crantz.) is one of largest carbohydrate sources in the world which also potentially developed as the source of bio-energy and raw materials for severals industries. As the demand for cassava is increasing, it is very important to supply the true-to-type cassava seedling continuously. In vitro propagation is one advanced technique that can be applied to meet the increasing demand of cassava seedling. The objective of the research was to study the effect of kinetin and Calcium Pantothenate (CaP) concentration on shoot multiplication of in vitro cultured cassava var. Adira 2 and Adira 4. This research was conducted in a completely randomized design with two factors. The first factor was kinetin concentration (0; 1; 1.5; and 3 ppm) and the second factor was concentration of CaP (0; 1; and 2 ppm) used in combination. The results showed that MS medium supplemented with 1 ppm CaP and 1.5 ppm kinetin promoted the growth of cassava explants of Adira 2 variety until 4 Weeks After Treatment (WAT). However, for longer culture period MS medium containing 3 ppm of BAP was better to support the explants growth. MS Medium containing 1 ppm of CaP and 3 ppm of kinetin promoted the growth of Adira 4 variety until 4 WAT. However, for longer culture period MS medium containing 2 ppm of CaP and 3 ppm of kinetin was better to support the explants growth. The highest shoot multiplication rate for 20 weeks old plantlets of Adira 2 and Adira 4 varieties was obtained at MS medium containing 3 ppm of BAP.
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Claims 5
Claim · model-written summary
CaP and kinetin promoted Adira 2 explant growth through 4 weeks.
- Taxon
- cassava
Manihot esculentae0 - Material
- Adira 2e0
- Treatment
- 1 ppm CaP and 1.5 ppm kinetine0
- Outcome
- growth of cassava explantse0
- Direction
- promotede0
- Qualifier
- until 4 Weeks After Treatment (WAT)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1 ppm CaP and 1.5 ppm kinetin promoted the growth of cassava explants of Adira 2 variety until 4 Weeks After Treatment (WAT).”
Claim · model-written summary
BAP better supported Adira 2 explant growth over longer culture.
- Taxon
- cassavaManihot esculentat0
- Material
- Adira 2t0
- Treatment
- 3 ppm of BAPe0
- Outcome
- explants growthe0
- Direction
- bettere0
- Qualifier
- longer culture periode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, for longer culture period MS medium containing 3 ppm of BAP was better to support the explants growth.”
t0“cassava var. Adira 2 and Adira 4”
Claim · model-written summary
CaP and kinetin promoted Adira 4 explant growth through 4 weeks.
- Taxon
- cassavaManihot esculentat0
- Material
- Adira 4e0
- Treatment
- 1 ppm of CaP and 3 ppm of kinetine0
- Outcome
- growthe0
- Direction
- promotede0
- Qualifier
- until 4 WATe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS Medium containing 1 ppm of CaP and 3 ppm of kinetin promoted the growth of Adira 4 variety until 4 WAT.”
t0“cassava var. Adira 2 and Adira 4”
Claim · model-written summary
A different CaP concentration better supported Adira 4 growth over longer culture.
- Taxon
- cassavaManihot esculentat0
- Material
- Adira 4t0
- Treatment
- 2 ppm of CaP and 3 ppm of kinetine0
- Outcome
- explants growthe0
- Direction
- bettere0
- Qualifier
- longer culture periode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, for longer culture period MS medium containing 2 ppm of CaP and 3 ppm of kinetin was better to support the explants growth.”
t0“cassava var. Adira 2 and Adira 4”
Claim · model-written summary
BAP medium gave the highest shoot multiplication rate for both varieties at 20 weeks.
- Taxon
- cassavaManihot esculentat0
- Material
- Adira 2 and Adira 4 varietiese0
- Treatment
- 3 ppm of BAPe0
- Outcome
- shoot multiplication ratee0
- Direction
- higheste0
- Qualifier
- 20 weeks old plantletse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest shoot multiplication rate for 20 weeks old plantlets of Adira 2 and Adira 4 varieties was obtained at MS medium containing 3 ppm of BAP.”
t0“cassava var. Adira 2 and Adira 4”
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