Abstract
During the different growth stages of Cassava explants, the effects of carbon sources andgrowth regulators with different concentrations and combinations on the development of secondaryembryoids and plant regeneration were investigated with an approach based on the cyclicembryogenesis liquid culture system. The highest embryoid production i. e., 29.3 maturationembryoids per explant, was obtained from initial embryoids being maturated on the solid medium(MS medium supplemented with 0.1 mg / L BAP) for 15 d as explants. When maltose (40 g / L) wassubstituted for sucrose as carbon source in the culture medium, there was an increasing Potential bothin the efficiency of embryoid development (32.5 mature embryoids Per explant) as well as the frequency of plant regeneration (74.3%). An increase of 38.5% to 77.6% regeneration frequency was demonstrated when 2,4-D and PP,3,(0. 1 mg / L ) were contained in the culture in the culture medium. However, with a combination of 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L), a considerablereduction both in embryoid induction and frequency of plant regeneration was observed.
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Claims 6
Claim · model-written summary
Initial embryoids matured for 15 days on solid MS medium with BAP gave the highest embryoid production, 29.3 per explant.
- Taxon
- Cassava
Manihot esculentat0 - Treatment
- MS medium supplemented with 0.1 mg / L BAPe0
- Outcome
- embryoid productione0
- Direction
- higheste0
- Value
- 29.3 maturationembryoids per explante0
- Qualifier
- for 15 de0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest embryoid production i. e., 29.3 maturationembryoids per explant, was obtained from initial embryoids being maturated on the solid medium(MS medium supplemented with 0.1 mg / L BAP) for 15 d as explants.”
t0“Cassava”
Claim · model-written summary
Replacing sucrose with 40 g/L maltose increased mature embryoid development to 32.5 per explant.
- Taxon
- CassavaManihot esculentat0
- Treatment
- maltose (40 g / L)e0
- Outcome
- efficiency of embryoid developmente0
- Direction
- increasinge0
- Value
- 32.5 mature embryoids Per explante0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When maltose (40 g / L) wassubstituted for sucrose as carbon source in the culture medium, there was an increasing Potential bothin the efficiency of embryoid development (32.5 mature embryoids Per explant) as well as the frequency of plant regeneration (74.3%).”
t0“Cassava”
Claim · model-written summary
Replacing sucrose with maltose increased plant regeneration frequency to 74.3%.
- Taxon
- CassavaManihot esculentat0
- Treatment
- maltose (40 g / L)e0
- Outcome
- frequency of plant regeneratione0
- Direction
- increasinge0
- Value
- 74.3%e0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When maltose (40 g / L) wassubstituted for sucrose as carbon source in the culture medium, there was an increasing Potential bothin the efficiency of embryoid development (32.5 mature embryoids Per explant) as well as the frequency of plant regeneration (74.3%).”
t0“Cassava”
Claim · model-written summary
A medium containing 2,4-D and PP,3 increased regeneration frequency from 38.5% to 77.6%.
- Taxon
- CassavaManihot esculentat0
- Treatment
- 2,4-D and PP,3,(0. 1 mg / L )e0
- Outcome
- regeneration frequencye0
- Direction
- increasee0
- Value
- 38.5% to 77.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase of 38.5% to 77.6% regeneration frequency was demonstrated when 2,4-D and PP,3,(0. 1 mg / L ) were contained in the culture in the culture medium.”
t0“Cassava”
Claim · model-written summary
Combining 2,4-D with BAP considerably reduced embryoid induction.
- Taxon
- CassavaManihot esculentat0
- Treatment
- 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L)e0
- Outcome
- embryoid inductione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with a combination of 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L), a considerablereduction both in embryoid induction and frequency of plant regeneration was observed.”
t0“Cassava”
Claim · model-written summary
Combining 2,4-D with BAP or kinetin considerably reduced plant regeneration frequency.
- Taxon
- CassavaManihot esculentat0
- Treatment
- 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L)e0
- Outcome
- frequency of plant regeneratione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with a combination of 2,4-D and either BAP (2mg / L) or kinetin (2.0 mg / L), a considerablereduction both in embryoid induction and frequency of plant regeneration was observed.”
t0“Cassava”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.