Abstract
Abstract Two different protocols for in vitro regeneration of cassava using zygotic embryos and nodal axillary meristems have been developed. In both cases, buds were regenerated directly from excised explants without an intervening callus phase after a two‐step culture procedure. In cotyledonary explants derived from zygotic embryos, prolific shoot formation occurred within 2—3 weeks on MS medium supplemented with 0.5—5 mg/1 BAP alone or in combination with 0.1 mg/1 NAA. Nodal explants with axillary meristems derived from aseptically grown seedlings or stem cuttings were used to initiate a round compact bulb‐like structure on MS medium containing 10 mg/1 BAP. These latter structures, when cultured on MS medium supplemented with 0.1 mg/1 NAA, 1 mg/1 BAP and 0.1 mg/1 GA 3 , produced multiple shoots. Somatic embryos isolated at the globular/torpedo stage from zygotic embryo explants were also capable of multiple shoot production on medium with 1 mg/1 BAP. Rooting of regenerated shoots exceeded 95 % in phytohormone‐free MS medium. No change in their ploidy levels was observed. Therefore, the protocols developed should be of use in the particle gun and Agrobacterium ‐mediated genetic transformation of cassava.
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Claims 9
Claim · model-written summary
Two cassava in vitro regeneration protocols were developed.
- Taxon
- cassava
Manihot esculentae0 - Outcome
- in vitro regeneratione0
- Value
- Two different protocolse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two different protocols for in vitro regeneration of cassava using zygotic embryos and nodal axillary meristems have been developed”
Claim · model-written summary
Buds regenerated directly from excised explants without callus in both protocols.
- Taxon
- CassavaManihot esculentat0
- Outcome
- buds were regenerated directly from excised explants without an intervening callus phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In both cases, buds were regenerated directly from excised explants without an intervening callus phase”
t0“Cassava”
Claim · model-written summary
Cotyledonary explants formed prolific shoots within 2—3 weeks under BAP medium.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- cotyledonary explantse0
- Treatment
- MS medium supplemented with 0.5—5 mg/1 BAPe0
- Outcome
- prolific shoot formatione0
- Value
- 2—3 weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cotyledonary explants derived from zygotic embryos, prolific shoot formation occurred within 2—3 weeks on MS medium supplemented with 0.5—5 mg/1 BAP alone or in combination with 0.1 mg/1 NAA”
t0“Cassava”
Claim · model-written summary
Nodal explants formed compact bulb-like structures on BAP medium.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- Nodal explants with axillary meristemse0
- Treatment
- MS medium containing 10 mg/1 BAPe0
- Outcome
- round compact bulb‐like structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nodal explants with axillary meristems derived from aseptically grown seedlings or stem cuttings were used to initiate a round compact bulb‐like structure on MS medium containing 10 mg/1 BAP”
t0“Cassava”
Claim · model-written summary
Nodal structures produced multiple shoots on NAA, BAP and GA3 medium.
- Taxon
- CassavaManihot esculentat0
- Treatment
- MS medium supplemented with 0.1 mg/1 NAA, 1 mg/1 BAP and 0.1 mg/1 GA 3e0
- Outcome
- produced multiple shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These latter structures, when cultured on MS medium supplemented with 0.1 mg/1 NAA, 1 mg/1 BAP and 0.1 mg/1 GA 3 , produced multiple shoots”
t0“Cassava”
Claim · model-written summary
Somatic embryos from zygotic explants produced multiple shoots on BAP medium.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- Somatic embryos isolated at the globular/torpedo stagee0
- Treatment
- medium with 1 mg/1 BAPe0
- Outcome
- multiple shoot productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos isolated at the globular/torpedo stage from zygotic embryo explants were also capable of multiple shoot production on medium with 1 mg/1 BAP”
t0“Cassava”
Claim · model-written summary
Over 95% of regenerated shoots rooted in hormone-free MS medium.
- Taxon
- CassavaManihot esculentat0
- Organ / stage
- regenerated shootse0
- Treatment
- phytohormone‐free MS mediume0
- Outcome
- Rootinge0
- Direction
- exceedede0
- Value
- 95 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting of regenerated shoots exceeded 95 % in phytohormone‐free MS medium”
t0“Cassava”
Claim · model-written summary
Regenerated cassava shoots showed no ploidy level change.
- Taxon
- CassavaManihot esculentat0
- Outcome
- ploidy levelse0
- Direction
- No changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No change in their ploidy levels was observed”
t0“Cassava”
Claim · model-written summary
Authors recommend these protocols for cassava genetic transformation.
- Taxon
- cassavaManihot esculentae0
- Outcome
- particle gun and Agrobacterium ‐mediated genetic transformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protocols developed should be of use in the particle gun and Agrobacterium ‐mediated genetic transformation of cassava”
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See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.