Abstract
A study on rapid propagation of cassava through tissue culture was conducted with three elite cassava genotypes: Slicass 6, Slicass 11 and Cocoa from Sierra-Leone. They showed slow growth in Murashige & Skoog (MS) basal medium which was proven to be optimal for a vast number of cassava accessions. Prior to mutation induction, a large population needs to be produced for mutagen susceptibility test and for mutant population development. The ultimate objective of this study was to investigate the effects of plant growth regulators on the shoot development of three cassava genotypes. In vivo shoot tips were sterilized and sub-cultured on MS media supplemented with six combinations of plant growth regulators (PGRs) at different concentrations. The results showed that from all media used, the MS medium with 1.0 mg/L α-naphthalene acetic acid (NAA) showed the best response for rooting (5.50), fresh weight (0.29 g), root number (10.00) and plantlet height (3.81 cm), while 0.1 mg/L 6-benzylaminopurine (BAP) was found to be more favourable to shoot development of leaves (6.38). The highest plant height and fresh weight were 3.81 cm and 0.29 g, respectively for Cocoa at 1.0 mg/L α-naphthalene acetic acid (NAA), 10.00 roots for Slicass 6 at 1.0 mg/L, 6.37 leaf numbers for Slicass 11 at 0.1 mg/L 6-benzylaminopurine (BAP) and 5.6 at 1.0 and 1.5 mg/L α-naphthalene acetic acid (NAA). These observations indicate that a supplement of 0.1 mg/L 6-benzylaminopurine (BAP) in MS medium can be useful in propagation of recalcitrant cassava and low concentration of α-naphthalene acetic acid (NAA) will be beneficial in root induction prior to acclimatization with promotion in recovery of the ex vitro plants before field assessment. Key words: Cassava, Manihot esculenta, propagation, shoot tip, 6-benzylaminopurine (BAP), α-naphthalene acetic acid (NAA).
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Claims 13
Claim · model-written summary
The three cassava genotypes grew slowly on basal MS medium.
- Taxon
- cassava
Manihot esculentat0 - Material
- Slicass 6, Slicass 11 and Cocoae1
- Treatment
- Murashige & Skoog (MS) basal mediume0
- Outcome
- slow growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They showed slow growth in Murashige & Skoog (MS) basal medium”
t0“cassava (Manihot esculenta Crantz)”
e1“three elite cassava genotypes: Slicass 6, Slicass 11 and Cocoa”
Claim · model-written summary
MS with 1.0 mg/L NAA gave the best rooting response of 5.50.
- Taxon
- cassavaManihot esculentat0
- Treatment
- MS medium with 1.0 mg/L α-naphthalene acetic acid (NAA)e0
- Outcome
- rootinge0
- Direction
- beste0
- Value
- 5.50e0
- Comparator
- all media usede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium with 1.0 mg/L α-naphthalene acetic acid (NAA) showed the best response for rooting (5.50)”
t0“Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone”
e1“from all media used”
Claim · model-written summary
MS with 1.0 mg/L NAA gave fresh weight of 0.29 g.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 1.0 mg/L α-naphthalene acetic acid (NAA)e1
- Outcome
- fresh weighte0
- Value
- 0.29 ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fresh weight (0.29 g)”
t0“cassava”
e1“MS medium with 1.0 mg/L α-naphthalene acetic acid (NAA) showed the best response for rooting (5.50)”
Claim · model-written summary
MS with 1.0 mg/L NAA gave 10.00 roots.
- Taxon
- cassavaManihot esculentat0
- Outcome
- root numbere0
- Value
- 10.00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root number (10.00)”
t0“cassava”
Claim · model-written summary
MS with 1.0 mg/L NAA gave plantlet height of 3.81 cm.
- Taxon
- cassavaManihot esculentat0
- Outcome
- plantlet heighte0
- Value
- 3.81 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantlet height (3.81 cm)”
t0“cassava”
Claim · model-written summary
BAP at 0.1 mg/L favored shoot leaf development, reported as 6.38.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 0.1 mg/L 6-benzylaminopurine (BAP)e0
- Outcome
- shoot development of leavese0
- Direction
- more favourablee0
- Value
- 6.38e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.1 mg/L 6-benzylaminopurine (BAP) was found to be more favourable to shoot development of leaves (6.38)”
t0“cassava”
Claim · model-written summary
Cocoa reached 3.81 cm height at 1.0 mg/L NAA.
- Taxon
- cassavaManihot esculentat0
- Material
- Cocoae0
- Treatment
- 1.0 mg/L α-naphthalene acetic acid (NAA)e0
- Outcome
- plant heighte0
- Direction
- higheste0
- Value
- 3.81 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest plant height and fresh weight were 3.81 cm and 0.29 g, respectively for Cocoa at 1.0 mg/L α-naphthalene acetic acid (NAA)”
t0“cassava”
Claim · model-written summary
Cocoa reached 0.29 g fresh weight at 1.0 mg/L NAA.
- Taxon
- cassavaManihot esculentat0
- Material
- Cocoae0
- Treatment
- 1.0 mg/L α-naphthalene acetic acid (NAA)e0
- Outcome
- fresh weighte0
- Direction
- higheste0
- Value
- 0.29 ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest plant height and fresh weight were 3.81 cm and 0.29 g, respectively for Cocoa at 1.0 mg/L α-naphthalene acetic acid (NAA)”
t0“cassava”
Claim · model-written summary
Slicass 6 produced 10.00 roots at 1.0 mg/L.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10.00 roots for Slicass 6 at 1.0 mg/L”
t0“cassava”
Claim · model-written summary
Slicass 11 produced 6.37 leaves at 0.1 mg/L BAP.
- Taxon
- cassavaManihot esculentat0
- Material
- Slicass 11e0
- Treatment
- 0.1 mg/L 6-benzylaminopurine (BAP)e0
- Outcome
- leaf numberse0
- Value
- 6.37e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6.37 leaf numbers for Slicass 11 at 0.1 mg/L 6-benzylaminopurine (BAP)”
t0“cassava”
Claim · model-written summary
Another reported leaf count was 5.6 at 1.0 and 1.5 mg/L NAA.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 1.0 and 1.5 mg/L α-naphthalene acetic acid (NAA)e0
- Outcome
- leaf numberse0
- Value
- 5.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6.37 leaf numbers for Slicass 11 at 0.1 mg/L 6-benzylaminopurine (BAP) and 5.6 at 1.0 and 1.5 mg/L α-naphthalene acetic acid (NAA)”
t0“cassava (Manihot esculenta Crantz)”
Claim · model-written summary
The authors recommend 0.1 mg/L BAP for propagation.
- Taxon
- cassavaManihot esculentae0
- Treatment
- 0.1 mg/L 6-benzylaminopurine (BAP)e0
- Outcome
- useful in propagation of recalcitrant cassavae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a supplement of 0.1 mg/L 6-benzylaminopurine (BAP) in MS medium can be useful in propagation of recalcitrant cassava”
Claim · model-written summary
The authors recommend low NAA concentration for root induction before acclimatization.
- Taxon
- cassavaManihot esculentat0
- Treatment
- low concentration of α-naphthalene acetic acid (NAA)e0
- Outcome
- beneficial in root induction prior to acclimatization with promotion in recovery of the ex vitro plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low concentration of α-naphthalene acetic acid (NAA) will be beneficial in root induction prior to acclimatization with promotion in recovery of the ex vitro plants before field assessment”
t0“cassava”
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