Abstract
In vitro micropropagation is a rapid method of multiplying healthy planting material to control Cassava mosaic disease (CMD), one of a major constraint to cassava production in Africa. However, some cassava varieties have a low propagation ratio under in vitro conditions. The main objective of this study was to improve the in vitro propagation rate of five difficult to grow, farmer-preferred cassava varieties using plant growth regulators. Microcuttings from in vitro plantlets of five recalcitrant cassava varieties (Agbablé 3, Ampong, Bayérè, Bocou 5, Olékanga) were evaluated for their capacity to rapidly regenerate plantlets. Time to root or leaf formation, number of nodes, number of roots, and the in vitro plantlet length were evaluated on nine culture media combinations. We found that among all the cassava varieties studied, the shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators. These two hormones evaluated were better for regeneration of leaves, nodes and elongation of in vitro plantlets with optimum concentration of 5 and 10 nM or thidiazuron, and 0.12 or 0.24 µM for KIN. A survival rate between 85-91% was recorded under tunnel conditions and the plantlets appeared to be morphologically normal. The information obtained during this study will be useful for mass multiplication programs of elite cassava varieties.
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Claims 8
Claim · model-written summary
Supplemented or half strength MS media gave shortest leaf formation time of 4 to 7 days.
- Taxon
- cassava
Manihot esculentae1 - Treatment
- kinetin and thidiazurone0
- Outcome
- leafe0
- Direction
- shorteste0
- Value
- 4 to 7 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
The same media gave shortest root formation time of 9 to 14 days.
- Taxon
- cassavaManihot esculentae1
- Treatment
- kinetin and thidiazurone0
- Outcome
- roote0
- Direction
- shorteste0
- Value
- 9 to 14 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Half strength MS without regulators also achieved shortest leaf formation.
- Taxon
- cassavaManihot esculentae1
- Treatment
- half-strength MS without these plant growth regulatorse0
- Outcome
- leafe0
- Direction
- shorteste0
- Value
- 4 to 7 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Half strength MS without regulators also achieved shortest root formation.
- Taxon
- cassavaManihot esculentae1
- Treatment
- half-strength MS without these plant growth regulatorse0
- Outcome
- roote0
- Direction
- shorteste0
- Value
- 9 to 14 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Thidiazuron at 5 and 10 nM improved leaf, node and plantlet elongation regeneration.
- Taxon
- cassavaManihot esculentae1
- Treatment
- 5 and 10 nM or thidiazurone0
- Outcome
- regeneration of leaves, nodes and elongation of in vitro plantletse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two hormones evaluated were better for regeneration of leaves, nodes and elongation of in vitro plantlets with optimum concentration of 5 and 10 nM or thidiazuron”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Kinetin at 0.12 or 0.24 µM improved regeneration.
- Taxon
- cassavaManihot esculentae1
- Treatment
- 0.12 or 0.24 µM for KINe0
- Outcome
- regeneration of leaves, nodes and elongation of in vitro plantletse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two hormones evaluated were better for regeneration of leaves, nodes and elongation of in vitro plantlets with optimum concentration of 5 and 10 nM or thidiazuron, and 0.12 or 0.24 µM for KIN”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Tunnel survival was 85-91%.
- Taxon
- cassavaManihot esculentae1
- Outcome
- survival ratee0
- Value
- 85-91%e0
- Setting
- tunnel conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A survival rate between 85-91% was recorded under tunnel conditions”
e1“five recalcitrant cassava varieties (Agbablé 3”
Claim · model-written summary
Regenerated plantlets appeared morphologically normal.
- Taxon
- cassavaManihot esculentae1
- Outcome
- morphologically normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plantlets appeared to be morphologically normal”
e1“five recalcitrant cassava varieties (Agbablé 3”
The same questions in other papers
kinetin · growth · Manihot esculenta (cassava)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- positive
2 papers more in this group
See all 6 papers →Thidiazuron · growth · Manihot esculenta (cassava)Results agree
2 papers in the same direction as this paper
- positive
- positive
class · growth · Manihot esculenta (cassava)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.