Abstract
Abstract An efficient system for Agrobacterium tumefaciensmediated transformation of Jatropha curcas was developed in this study. Several factors affecting the transformation efficiency were optimized, including the explant type, preculture and coculture periods, usage of acetosyringone and density of A. tumefaciens. Compared with other explants, 2-day precultured and cocultured hypocotyl explants showed a significant GUS transient expression efficiency (67.7%). Moreover, adding AS showed a remarkable increase in transformation efficiency. After infecting with A. tumefaciens, hypocotyl explants were subjected to expansion and proliferation on MS medium with 1.0 mg· l-1 IBA and 0.5 mg· l-1 BA. Transformants were demonstrated by the GUS assay and PCR analysis. Rate of hypocotyl explants forming resistant calli reached 67.08%. Furthermore, in the transformed calli development, morphologic changes (calli superficial development and internal structure) were observed by SEM and LM. The present study has provided a fundamental information and research approach for the future study of inducing new traits to J. curcas and genetic modification.
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Claims 6
Claim · model-written summary
An efficient Agrobacterium transformation system was developed for Jatropha.
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Outcome
- transformatione0
- Value
- efficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“An efficient system for Agrobacterium tumefaciensmediated transformation of Jatropha curcas was developed in this study”
Claim · model-written summary
Precultured and cocultured hypocotyl explants reached 67.7% GUS transient expression efficiency.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- hypocotyl explantse0
- Outcome
- GUS transient expression efficiencye0
- Value
- 67.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2-day precultured and cocultured hypocotyl explants showed a significant GUS transient expression efficiency (67.7%)”
t0“Jatropha curcas”
Claim · model-written summary
Adding acetosyringone increased transformation efficiency.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- adding ASe0
- Outcome
- transformation efficiencye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“adding AS showed a remarkable increase in transformation efficiency”
t0“Jatropha curcas”
Claim · model-written summary
The transformants were verified with GUS and PCR assays.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- Transformantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformants were demonstrated by the GUS assay and PCR analysis”
t0“Jatropha curcas”
Claim · model-written summary
Resistant calli formed from 67.08% of hypocotyl explants.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- hypocotyl explantse0
- Outcome
- forming resistant callie0
- Direction
- reachede0
- Value
- 67.08%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rate of hypocotyl explants forming resistant calli reached 67.08%”
t0“Jatropha curcas”
Claim · model-written summary
Transformed calli showed surface and internal morphological changes.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- callie0
- Outcome
- morphologic changes (calli superficial development and internal structure)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“morphologic changes (calli superficial development and internal structure) were observed by SEM and LM”
t0“Jatropha curcas”