Abstract
A reliable protocol for the regeneration of onion through repetitive somatic embryogenesis was established. Embryogenic callus was derived from mature seeds on Murashige and Skoog (MS) medium supplemented with 2 mg dm-3 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos aroused on the surface of calli cultures and formed plantlets after the removal of 2,4-D or its substitution with 1 mg dm-3 kinetin (Kin). Reculturing the somatic embryos on 2,4-D containing medium led to secondary embryos formation. The embryogenic cultures which were preserved for five months on maintenance medium containing 2 mg dm-3 2,4-D + 0.5 mg dm-3 Kin have retained their ability for regeneration, while those kept on 2,4-D only, failed to form plantlets. Electrophoretic analysis of total soluble proteins revealed that the competence for successful conversion of somatic embryos into plantlets is associated with the expression of new set of proteins (112, 58 and 30 kD). The regenerated plants were successfully transferred to the soil.
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Claims 8
Claim · model-written summary
An onion regeneration protocol through repetitive somatic embryogenesis was established.
- Taxon
- onion
Allium cepae0 - Outcome
- regeneration of onion through repetitive somatic embryogenesise0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reliable protocol for the regeneration of onion through repetitive somatic embryogenesis was established”
Claim · model-written summary
Removing 2,4-D allowed somatic embryos to form plantlets.
- Taxon
- onionAllium cepat0
- Treatment
- removal of 2,4-De0
- Outcome
- formed plantletse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos aroused on the surface of calli cultures and formed plantlets after the removal of 2,4-D or its substitution with 1 mg dm-3 kinetin (Kin)”
t0“onion”
Claim · model-written summary
Substitution with kinetin allowed somatic embryos to form plantlets.
- Taxon
- onionAllium cepat0
- Treatment
- substitution with 1 mg dm-3 kinetin (Kin)e0
- Outcome
- formed plantletse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos aroused on the surface of calli cultures and formed plantlets after the removal of 2,4-D or its substitution with 1 mg dm-3 kinetin (Kin)”
t0“onion”
Claim · model-written summary
Reculturing somatic embryos on 2,4-D medium produced secondary embryos.
- Taxon
- onionAllium cepat0
- Treatment
- 2,4-D containing mediume0
- Outcome
- secondary embryos formatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reculturing the somatic embryos on 2,4-D containing medium led to secondary embryos formation”
t0“onion”
Claim · model-written summary
Embryogenic cultures retained regeneration ability after five months on medium with 2,4-D and kinetin.
- Taxon
- onionAllium cepat0
- Treatment
- maintenance medium containing 2 mg dm-3 2,4-D + 0.5 mg dm-3 Kine0
- Outcome
- ability for regeneratione0
- Qualifier
- five monthse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryogenic cultures which were preserved for five months on maintenance medium containing 2 mg dm-3 2,4-D + 0.5 mg dm-3 Kin have retained their ability for regeneration”
t0“onion”
Claim · model-written summary
Cultures maintained on 2,4-D alone failed to form plantlets.
- Taxon
- onionAllium cepat0
- Treatment
- 2,4-D onlye0
- Outcome
- failed to form plantletse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those kept on 2,4-D only, failed to form plantlets”
t0“onion”
Claim · model-written summary
Embryo conversion competence was associated with expression of new 112, 58, and 30 kD proteins.
- Taxon
- onionAllium cepat0
- Gene
- new set of proteinse0
- Outcome
- competence for successful conversion of somatic embryos into plantletse0
- Qualifier
- 112, 58 and 30 kDe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the competence for successful conversion of somatic embryos into plantlets is associated with the expression of new set of proteins (112, 58 and 30 kD)”
t0“onion”
Claim · model-written summary
Regenerated onion plants transferred successfully to soil.
- Taxon
- onionAllium cepat0
- Outcome
- successfully transferred to the soile0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated plants were successfully transferred to the soil”
t0“onion”
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