Abstract
Salt stress elicits betaine accumulation to high levels in species from several diverse dicot families (Chenopodiaceae, Amaranthaceae, Convolvulaceae, Solanaceae, and Asteraceae). FAM-MS studies with deuterated precursors showed that species from all these families synthesize betaine from choline. Enzyme assays and immunotitration data showed that all accumulating species contained betaine aldehyde dehydrogenase (BADH) enzyme activity recognized by antibodies raised against purified BADH isolated from Spinacia oleracea. Immunoblotting indicated that the BADH monomer was in all cases of Mr {approx} 63,000. The similarity of BADH in the different species is consistent with a single evolutionary origin for the betaine pathway. This was supported by the presence in immunoblots of a cross-reacting band at Mr {approx} 63,000 in Magnolia x Soulangiana, a primitive angiosperm.
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Claims 5
Claim · model-written summary
Salt stress elicited high betaine accumulation in species from five dicot families.
Not reportedComparator
Verbatim
e0“Salt stress elicits betaine accumulation to high levels in species from several diverse dicot families (Chenopodiaceae, Amaranthaceae, Convolvulaceae, Solanaceae, and Asteraceae)”
Claim · model-written summary
The species synthesized betaine from choline.
Verbatim
e0“species from all these families synthesize betaine from choline”
Claim · model-written summary
All betaine accumulating species had BADH enzyme activity recognized by anti-spinach BADH antibodies.
Verbatim
e0“all accumulating species contained betaine aldehyde dehydrogenase (BADH) enzyme activity recognized by antibodies raised against purified BADH isolated from Spinacia oleracea”
Claim · model-written summary
The BADH monomer had molecular mass approximately 63,000 in all cases.
Verbatim
e0“the BADH monomer was in all cases of Mr {approx} 63,000”
t0“dicot families”
Claim · model-written summary
A cross-reacting band of approximately 63,000 was present in Magnolia x Soulangiana.
- Taxon
- Magnolia x Soulangiana
.jpg?width=60)
Magnolia ×soulangeanae0 - Outcome
- cross-reacting bande0
- Value
- {approx} 63,000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a cross-reacting band at Mr {approx} 63,000 in Magnolia x Soulangiana”