Paper · 1959 · Biologia Plantarum

The relation of the refractive index of plant cell sap to its osmotic pressure

Bohdan Slavík

6
claims
12
verbatim quotes
48
citations
3
research areas
—
Not open access

Abstract

Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species. At the same time both values were ascertained as well as their relation for aqueous solutions of some sugars, organic acids and inorganic salts. The relations found with these model solutions are different for different substances. The quantitative composition of osmotically active components of cell sap changes in the course of ontogenesis and as a result the relation of refractive index and osmotic pressure also changes. By this comparison it has been shown that a reliable and more or less linear relation of the two values, which is advantageous for practical application, is only valid for a limited period in the ontogenesis of the experimental plant. The determination of the osmotic pressure of cell sap from the refractive index, the main advantage of which is its quickness and the small volume of sap required (0-02 ml.), is reliable if we construct empiricallyad hoc the appropriate correlation graph for the experimental plant for a limited ontogenetic period.

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Claims 6

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The relation between refractive index and osmotic pressure differs between model solutions of different substances.

Outcome
relations found with these model solutionse1
Qualifier
different for different substancese1
Comparator
aqueous solutions of some sugars, organic acids and inorganic saltse2

Not reportedTaxonDirection

Verbatim

e1

“The relations found with these model solutions are different for different substances.”

✓ located verbatim in the source · abstract, characters 306–392 · show in text ↑
e2

“At the same time both values were ascertained as well as their relation for aqueous solutions of some sugars, organic acids and inorganic salts.”

✓ located verbatim in the source · abstract, characters 161–305 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

A reliable and roughly linear relation between refractive index and osmotic pressure holds only for a limited period in the ontogenesis of the experimental plant.

Taxon
experimental plante1
Outcome
reliable and more or less linear relation of the two valuese1
Qualifier
only valid for a limited period in the ontogenesise1
Comparator
cryoscopic methode2

Verbatim

e1

“By this comparison it has been shown that a reliable and more or less linear relation of the two values, which is advantageous for practical application, is only valid for a limited period in the ontogenesis of the experimental plant.”

✓ located verbatim in the source · abstract, characters 588–822 · show in text ↑
e2

“Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species.”

✓ located verbatim in the source · abstract, characters 0–160 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Osmotic pressure of cell sap can be determined from refractive index for the experimental plant over a limited ontogenetic period, given a correlation graph built for that plant.

Taxon
experimental plante1
Outcome
determination of the osmotic pressure of cell sap from the refractive indexe1
Qualifier
for a limited ontogenetic periode1
Comparator
cryoscopic methode2

Verbatim

e1

“The determination of the osmotic pressure of cell sap from the refractive index, the main advantage of which is its quickness and the small volume of sap required (0-02 ml.), is reliable if we construct empiricallyad hoc the appropriate correlation graph for the experimental plant for a limited ontogenetic period.”

✓ located verbatim in the source · abstract, characters 823–1138 · show in text ↑
e2

“Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species.”

✓ located verbatim in the source · abstract, characters 0–160 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Determining osmotic pressure from refractive index needs only a small sap volume of 0-02 ml.

Taxon
seven plant speciese1
Outcome
small volume of sap requirede2
Value
0-02 ml.e2

Verbatim

e1

“Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species.”

✓ located verbatim in the source · abstract, characters 0–160 · show in text ↑
e2

“The determination of the osmotic pressure of cell sap from the refractive index, the main advantage of which is its quickness and the small volume of sap required (0-02 ml.), is reliable if we construct empiricallyad hoc the appropriate correlation graph for the experimental plant for a limited ontogenetic period.”

✓ located verbatim in the source · abstract, characters 823–1138 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The quantitative composition of osmotically active components of cell sap changes in the course of ontogenesis.

Taxon
seven plant speciese2
Organ / stage
the course of ontogenesise1
Outcome
quantitative composition of osmotically active components of cell sape1
Direction
changese1

Verbatim

e1

“The quantitative composition of osmotically active components of cell sap changes in the course of ontogenesis and as a result the relation of refractive index and osmotic pressure also changes.”

✓ located verbatim in the source · abstract, characters 393–587 · show in text ↑
e2

“Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species.”

✓ located verbatim in the source · abstract, characters 0–160 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The relation between refractive index and osmotic pressure of cell sap changes during ontogenesis.

Taxon
seven plant speciese2
Organ / stage
the course of ontogenesise1
Outcome
relation of refractive index and osmotic pressuree1
Direction
changese1

Verbatim

e1

“The quantitative composition of osmotically active components of cell sap changes in the course of ontogenesis and as a result the relation of refractive index and osmotic pressure also changes.”

✓ located verbatim in the source · abstract, characters 393–587 · show in text ↑
e2

“Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species.”

✓ located verbatim in the source · abstract, characters 0–160 · show in text ↑