Paper · 1999 · Biologia Plantarum

Isoenzyme Expression During Root and Shoot Formation in Solanum Nigrum

A.M. Hassanein; A.M. Ahmed; A.I.I. Abed-El-Hafez; et al.

8
claims
14
verbatim quotes
7
citations
2
research areas
—
Not open access

Abstract

In Solanum nigrum, the initiation of root primordia occurred directly on stem explants or microshoots cultured on half strength MS medium supplemented with 1 mg dm-3 indolebutyric acid (IBA), indole-3-acetic acid, or naphthalene acetic acid, IBA being most effective. Initiation of shoot primordia occurred on B5 medium supplemented with 0.5 mg dm-3 benzylaminopurine (BAP) or kinetin; BAP was more active. During shoot formation, the increased content and the expression of new isoenzymes of peroxidase (POX), esterase and malate dehydrogenase was found. On the other side, expression of new indophenol peroxidase isoenzyme was detected during root formation. Organogenesis of S. nigrum has no effect on glutamate oxaloacetate transaminase and glutamate dehydrogenase content. Differential expression of POX could be detected between basal and upper side of explants.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Root primordia formed directly on stem explants or microshoots with auxin-supplemented medium.

Taxon
Solanum nigrumSolanum nigrume0
Organ / stage
stem explants or microshootse0
Outcome
initiation of root primordiae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In Solanum nigrum, the initiation of root primordia occurred directly on stem explants or microshoots cultured on half strength MS medium supplemented with 1 mg dm-3 indolebutyric acid (IBA), indole-3-acetic acid, or naphthalene acetic acid”

✓ located verbatim in the source · abstract, characters 0–240 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA was most effective for root primordium initiation.

Taxon
Solanum nigrumSolanum nigrumt0
Treatment
IBAe0
Outcome
most effectivee0
Direction
most effectivee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“IBA being most effective”

✓ located verbatim in the source · abstract, characters 242–266 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoot primordia formed with BAP or kinetin.

Taxon
Solanum nigrumSolanum nigrumt0
Outcome
Initiation of shoot primordiae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Initiation of shoot primordia occurred on B5 medium supplemented with 0.5 mg dm-3 benzylaminopurine (BAP) or kinetin”

✓ located verbatim in the source · abstract, characters 268–384 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP was more active for shoot primordia than kinetin.

Taxon
Solanum nigrumSolanum nigrumt0
Treatment
BAPe0
Outcome
more activee0
Direction
more activee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“BAP was more active”

✓ located verbatim in the source · abstract, characters 386–405 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoot formation increased peroxidase, esterase and malate dehydrogenase content and brought new isoenzymes.

Taxon
Solanum nigrumSolanum nigrumt0
Outcome
increased content and the expression of new isoenzymes of peroxidase (POX), esterase and malate dehydrogenasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“During shoot formation, the increased content and the expression of new isoenzymes of peroxidase (POX), esterase and malate dehydrogenase was found”

✓ located verbatim in the source · abstract, characters 407–554 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A new indophenol peroxidase isoenzyme was expressed during root formation.

Taxon
Solanum nigrumSolanum nigrumt0
Outcome
expression of new indophenol peroxidase isoenzymee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“expression of new indophenol peroxidase isoenzyme was detected during root formation”

✓ located verbatim in the source · abstract, characters 575–659 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Organogenesis did not affect glutamate oxaloacetate transaminase or glutamate dehydrogenase content.

Taxon
S. nigrumSolanum nigrume0
Outcome
glutamate oxaloacetate transaminase and glutamate dehydrogenase contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Organogenesis of S. nigrum has no effect on glutamate oxaloacetate transaminase and glutamate dehydrogenase content”

✓ located verbatim in the source · abstract, characters 661–776 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

POX expression differed between basal and upper explant sides.

Taxon
Solanum nigrumSolanum nigrumt0
Outcome
Differential expression of POXe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum nigrum
Solanum nigrum · TaxonHladac · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Differential expression of POX could be detected between basal and upper side of explants”

✓ located verbatim in the source · abstract, characters 778–867 · show in text ↑
t0

“Solanum Nigrum”

✓ located verbatim in the source · title, characters 56–70 · show in text ↑