Abstract
The NPR1 protein of Arabidopsis thaliana has been shown to be an important regulatory component of systemic acquired resistance (SAR). Mutations in the NPR1 gene block the induction of SAR by the signal molecule salicylic acid (SA). NPR1 contains an ankyrin repeats and a BTB domain which are involved in interaction with other protein(s). To further study the function of NPR1 and the regulatory mechanism of SAR, we used both molecular and genetic approaches to identify additional SAR regulatory components. Through a yeast two-hybrid screen we found that NPR1 interacts specifically with bZIP transcription factors. The involvement of bZIP transcription factors in controlling the SA-induced genes had been suggested by a number of promoter studies performed on these genes. It was found that as1 element, which is a binding site for bZIP transcription factors, is essential for SA-induced gene expression. In a genetic screen for suppressors of npr1, we found a mutant, sni1, that restored the responsiveness to SAR induction in npr1. The genetic characteristics of the sni1 mutant and the sequence of SNI1 suggest that the wild-type SNI1 protein is a negative regulator of SAR. We believe that SAR is controlled by both positive regulators and negative regulators.
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Claims 6
Claim · model-written summary
NPR1 mutations blocked salicylic acid induction of systemic acquired resistance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Mutations in the NPR1 genee0
- Gene
- NPR1 genee0
- Compound
- salicylic acide0
- Outcome
- induction of SARe0
- Direction
- blocke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NPR1 protein of Arabidopsis thaliana has been shown to be an important regulatory component of systemic acquired resistance (SAR). Mutations in the NPR1 gene block the induction of SAR by the signal molecule salicylic acid (SA)”
Claim · model-written summary
NPR1 specifically interacted with bZIP transcription factors.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- NPR1e1
- Outcome
- NPR1 interacts specifically with bZIP transcription factorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPR1 protein of Arabidopsis thaliana”
e1“we found that NPR1 interacts specifically with bZIP transcription factors”
Claim · model-written summary
The as1 element was essential for salicylic acid induced gene expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- as1 elemente1
- Outcome
- SA-induced gene expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPR1 protein of Arabidopsis thaliana”
e1“as1 element, which is a binding site for bZIP transcription factors, is essential for SA-induced gene expression”
Claim · model-written summary
The sni1 mutant restored responsiveness to SAR induction in npr1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- sni1e1
- Outcome
- responsiveness to SAR induction in npr1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPR1 protein of Arabidopsis thaliana”
e1“we found a mutant, sni1, that restored the responsiveness to SAR induction in npr1”
Claim · model-written summary
The authors infer that wild-type SNI1 negatively regulates systemic acquired resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- SNI1 proteine1
- Outcome
- negative regulator of SARe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPR1 protein of Arabidopsis thaliana”
e1“the wild-type SNI1 protein is a negative regulator of SAR”
Claim · model-written summary
The authors infer that both positive and negative regulators control systemic acquired resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- SAR is controllede1
- Qualifier
- both positive regulators and negative regulatorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPR1 protein of Arabidopsis thaliana”
e1“SAR is controlled by both positive regulators and negative regulators”
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