Abstract
Proline plays important role in osmo-adjusment and prevention of osmotic stress in plants. In order to analyze osmotic stress resistance, germination and growth pattern in vitro and in vivo, the Arabidopsis thalianaδ-1-pyrroline-5-carboxylate synthetase (P5CS) which is the regulatory enzyme of proline biosynthesis, was reverse transcribed, amplified into double stranded cDNA, cloned and transformed into tobacco using Agrobacterium tumefaciens mediated transformation. Seeds of chimeric T0 transgenic plants were grown; T1 and the succeeding T2 transgenic plants were obtained. Subsequently, wild type and binary vector transformed control plants along with T2 transgenic lines were exposed to salt concentrations from 0 to 250 mM during germination and growth stages. Results showed a significant difference (P < 0.0001) between T2 and control plants, while the control plants germinated in the presence of salt concentrations up to 50 mM and tolerated 100 mM salt during growth phase. Transgenic T2 lines were able to germinate in 200 mM NaCl and tolerated up to 250 mM salt during growth phase. Assessing proline contents, we observed 7.5 times more proline in T2 transgenic plants than control plants in normal condition which increased up to 3.3 folds in stress condition. This osmotic stress resistance was further assessed for T3 generation against drought and salt stresses in vivo. Results indicated osmotic stress resistance of T3generation as well as stable expression of P5CS along with achievement of homozygous transgenic lines. Key words: δ-1-Pyrroline-5-carboxylate synthetase (P5CS), osmotic stress, transgenic plants, germination, growth phase.
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Claims 10
Claim · model-written summary
T2 transgenic tobacco differed significantly from controls under salt exposure.
- Taxon
- tobacco
Nicotiana tabacume1 - Material
- T2e0
- Treatment
- salt concentrations from 0 to 250 mMe2
- Outcome
- significant differencee0
- Comparator
- control plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results showed a significant difference (P < 0.0001) between T2 and control plants”
e1“transformed into tobacco”
e2“exposed to salt concentrations from 0 to 250 mM”
Claim · model-written summary
Controls germinated with salt concentrations up to 50 mM.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- control plantse0
- Treatment
- salt concentrations up to 50 mMe0
- Outcome
- germinatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the control plants germinated in the presence of salt concentrations up to 50 mM”
e1“transformed into tobacco”
Claim · model-written summary
Controls tolerated 100 mM salt during growth.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- control plantse1
- Outcome
- tolerated 100 mM salte0
- Value
- 100 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerated 100 mM salt during growth phase”
e1“the control plants germinated”
e2“transformed into tobacco”
Claim · model-written summary
T2 transgenic lines germinated in 200 mM NaCl.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- Transgenic T2 linese0
- Outcome
- germinatee0
- Value
- 200 mM NaCle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic T2 lines were able to germinate in 200 mM NaCl”
e1“transformed into tobacco”
Claim · model-written summary
T2 transgenic lines tolerated up to 250 mM salt during growth.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- Transgenic T2 linese1
- Outcome
- tolerated up to 250 mM salte0
- Value
- 250 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerated up to 250 mM salt during growth phase”
e1“Transgenic T2 lines were able”
e2“transformed into tobacco”
Claim · model-written summary
T2 transgenic plants had 7.5 times more proline than controls under normal conditions.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- T2 transgenic plantse0
- Outcome
- prolinee0
- Direction
- moree0
- Value
- 7.5 timese0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7.5 times more proline in T2 transgenic plants than control plants in normal condition”
e1“transformed into tobacco”
Claim · model-written summary
Under stress, proline in T2 transgenic plants increased up to 3.3 folds.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- T2 transgenic plantse1
- Outcome
- prolinee1
- Direction
- increasede0
- Value
- 3.3 foldse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which increased up to 3.3 folds in stress condition”
e1“proline in T2 transgenic plants than control plants”
e2“transformed into tobacco”
Claim · model-written summary
T3 generation showed osmotic stress resistance against drought and salt in vivo.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- T3 generatione0
- Treatment
- drought and salt stressese0
- Outcome
- osmotic stress resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This osmotic stress resistance was further assessed for T3 generation against drought and salt stresses in vivo. Results indicated osmotic stress resistance of T3generation”
e1“transformed into tobacco”
Claim · model-written summary
T3 plants had stable expression of P5CS.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- P5CSe0
- Outcome
- stable expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of P5CS”
e1“transformed into tobacco”
Claim · model-written summary
Homozygous transgenic lines were obtained.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- homozygous transgenic linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“achievement of homozygous transgenic lines”
e1“transformed into tobacco”