Abstract
Soybean is an important crop because its seed has very high protein relative to others, The quality of soy protein is limited by the concentration of the sulfur-containing amino acids in the amino acid profile. Among the supply of various forms of 0.4mM sulfur as S nutrition during seed fill, only 0.4mM L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds. Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration. Supply of 0.2mM cystine (0.4mM S) did not affect the accumulative pattern of seed storage protein (SSP) subunits. In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than the sulfate control. The relative abundance of α'-subunit was not altered by any N or S nutrition. Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of S nutrition. This increase resulted in the only increase of 7S protein, mainly β-subunit. Our data suggest that the regulatory system of SSP genes responds to the balance between N and S assimilates supplied from mother plant, and controls the differential synthesis of their subunits for the maximum protein accumulation in developing soybean seed.
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Claims 11
Claim · model-written summary
0.4mM L-methionine completely inhibited β-subunit synthesis.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- 0.4mM L-methioninee0
- Outcome
- β-subunit synthesise0
- Direction
- inhibite0
- Comparator
- 0.4mM sulfate controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 0.4mM L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds”
t0“Soybean Seed”
e1“Compared to 0.4mM sulfate control”
Claim · model-written summary
0.4mM L-methionine produced the highest glycinin-containing seeds.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.4mM L-methioninee0
- Outcome
- glycinin-containing seedse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 0.4mM L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds”
t0“Soybean Seed”
Claim · model-written summary
Methionine lowered α-subunit abundance versus sulfate.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.4mM L-methioninee0
- Outcome
- α-e0
- Direction
- lowere0
- Comparator
- 0.4mM sulfate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration”
t0“Soybean Seed”
Claim · model-written summary
Methionine increased glycinin versus sulfate.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.4mM L-methioninee0
- Outcome
- glycinine0
- Direction
- increasede0
- Comparator
- 0.4mM sulfate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration”
t0“Soybean Seed”
Claim · model-written summary
Methionine did not alter total protein concentration.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.4mM L-methioninee0
- Outcome
- total protein concentratione0
- Direction
- without alteringe0
- Comparator
- 0.4mM sulfate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration”
t0“Soybean Seed”
Claim · model-written summary
0.2mM cystine did not affect seed storage protein subunit accumulation.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.2mM cystine (0.4mM S)e0
- Outcome
- accumulative pattern of seed storage protein (SSP) subunitse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supply of 0.2mM cystine (0.4mM S) did not affect the accumulative pattern of seed storage protein (SSP) subunits”
t0“Soybean Seed”
Claim · model-written summary
0.2mM methionine raised seed glycinin versus sulfate.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.2mM treatmente0
- Outcome
- glycinin in seedse0
- Direction
- highere0
- Comparator
- sulfate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than the sulfate control”
t0“Soybean Seed”
Claim · model-written summary
0.05mM methionine lowered seed glycinin versus sulfate.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 0.05mMe0
- Outcome
- glycinine0
- Direction
- lowere0
- Comparator
- sulfate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than the sulfate control”
t0“Soybean Seed”
Claim · model-written summary
No nitrogen or sulfur treatment altered α′-subunit relative abundance.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- any N or S nutritione0
- Outcome
- relative abundance of α'-subunite0
- Direction
- not alterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative abundance of α'-subunit was not altered by any N or S nutrition”
t0“Soybean Seed”
Claim · model-written summary
Replacing nitrate with ammonia at 5mM nitrogen increased protein concentration about 3–5%.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- substituting ammoniae0
- Outcome
- protein concentratione0
- Direction
- increasede0
- Value
- about 3-5%e0
- Comparator
- nitratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of S nutrition”
t0“Soybean Seed”
Claim · model-written summary
The protein gain from ammonia substitution was in 7S protein, mainly β-subunit.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- substituting ammoniae2
- Outcome
- 7S protein, mainly β-subunite0
- Direction
- increasee0
- Comparator
- nitratee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increase resulted in the only increase of 7S protein, mainly β-subunit”
t0“Soybean Seed”
e2“Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of S nutrition”
The same questions in other papers
sulfur atom · pest · Glycine max (soybean)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
L-methionine · quality: protein · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.