Abstract
The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolation. STS also decreased the internode length of potato shoots but increased dry weight and chlorophyll. Regenerated plants were obtained from cultured protoplasts in a series of media based on the MS medium. Protoplasts of Delaware showed a better response in cell division and colony formation in agarose-solidified culture medium. Regenerated plants showed some degrees of aneuploid but basically were similar to the original potato plants.
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Claims 8
Claim · model-written summary
Ethylene accumulation reduced leaf surface, dry weight and chlorophyll in shoot cultures.
- Taxon
- potato
Solanum tuberosumt0 - Compound
- Ethylenee0
- Outcome
- leaf surface, dry weight and chlorophylle0
- Direction
- decreasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture”
t0“potato”
Claim · model-written summary
Silver thiosulfate enlarged leaf area for protoplast sourcing.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Fifty to one hundred μM Silver thiosulfate (STS)e0
- Outcome
- leaf areae0
- Direction
- largere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifty to one hundred μM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolation”
t0“potato”
Claim · model-written summary
STS shortened potato shoot internodes.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- STSe0
- Outcome
- internode lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“STS also decreased the internode length of potato shoots but increased dry weight and chlorophyll”
Claim · model-written summary
STS increased potato dry weight and chlorophyll.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- STSe0
- Outcome
- dry weight and chlorophylle0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“STS also decreased the internode length of potato shoots but increased dry weight and chlorophyll”
Claim · model-written summary
Plants regenerated from cultured protoplasts in MS-based media.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- media based on the MS mediume0
- Outcome
- Regenerated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants were obtained from cultured protoplasts in a series of media based on the MS medium”
t0“potato”
Claim · model-written summary
Delaware protoplasts divided and formed colonies better in agarose-solidified medium.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Delawaree0
- Treatment
- agarose-solidified culture mediume0
- Outcome
- cell division and colony formatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts of Delaware showed a better response in cell division and colony formation in agarose-solidified culture medium”
t0“potato”
Claim · model-written summary
Regenerated plants showed some aneuploidy.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- some degrees of aneuploide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants showed some degrees of aneuploid but basically were similar to the original potato plants”
Claim · model-written summary
Regenerated plants were basically similar to the original potato plants.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- similar to the original potato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants showed some degrees of aneuploid but basically were similar to the original potato plants”
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1 paper more in this group
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2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.