Paper · 2010 · http://jast.modares.ac.ir/pdf_4365_62aeb679d42a5b5bcb5c5b9494499a22.html

Optimizing Storage and In vitro Germination of Date Palm (Phoenix dactylifera) Pollen

Seyed Mohammad Hassan Mortazavi; K. Arzani; A. A. Moini

7
claims
13
verbatim quotes
34
citations
4
research areas
—
Not open access

Abstract

Iran is one of the main date (Phoenix dactylifera) producing countries in the world. however, little information is available on the pollen viability and favorable storage conditions of Iranian male date cultivars. This study was conducted to estimate the best in vitro pollen germination of three male date palm cultivars, `Ghanami’, `Samsmavi ’ and `Gheibani ’ using various doses of boric acid (0, 50, 100 and 200 mg L-1), calcium nitrate (0, 200 and 300 mg L-1) and sucrose (15 % and 25 % (w/v)) in a Modified Brebaker and Kwack (MBK) medium. The results showed that the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose. Once the optimal in vitro germination medium had been established, pollen viability ( % germination) of these three cultivars stored for up to 200 days at room temperature (dark and light), in a refrigerator (4˚C), freezer (-20˚C) and in liquid nitrogen (-196˚C) was compared. For all the tested cultivars, germination declined as storage temperature increased and, by 200 days, the highest survival rate was obtained with cryostored pollen. Long-term storage of date pollen using an ultra-low temperature (-196˚C) can be used without any deteriorating effect on pollen viability, while room temperature storage significantly reduced pollen germination.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Best in vitro pollen germination occurred with 50 mg L-1 boric acid.

Taxon
Date PalmPhoenix dactyliferat0
Organ / stage
pollent0
Treatment
50 mg L-1 boric acide0
Outcome
germinatione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”

✓ located verbatim in the source · abstract, characters 571–681 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Best in vitro pollen germination occurred with 200 mg L-1 calcium nitrate.

Taxon
Date PalmPhoenix dactyliferat0
Organ / stage
pollent0
Treatment
200 mg L-1 calcium nitratee0
Outcome
germinatione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”

✓ located verbatim in the source · abstract, characters 571–681 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Best in vitro pollen germination occurred with 15 % (w/v) sucrose.

Taxon
Date PalmPhoenix dactyliferat0
Organ / stage
pollent0
Treatment
15 % (w/v) sucrosee0
Outcome
germinatione0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”

✓ located verbatim in the source · abstract, characters 571–681 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Pollen germination declined as storage temperature increased across all tested cultivars.

Taxon
Date PalmPhoenix dactyliferat0
Material
all the tested cultivarse0
Organ / stage
Pollent0
Treatment
storage temperaturee0
Outcome
germinatione0
Direction
declinede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“For all the tested cultivars, germination declined as storage temperature increased”

✓ located verbatim in the source · abstract, characters 960–1043 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cryostored pollen had the highest survival by 200 days.

Taxon
Date PalmPhoenix dactyliferat0
Organ / stage
pollene0
Treatment
cryostorede0
Outcome
survival ratee0
Direction
higheste0
Qualifier
by 200 dayse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“by 200 days, the highest survival rate was obtained with cryostored pollen”

✓ located verbatim in the source · abstract, characters 1049–1123 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Long-term storage at -196˚C did not deteriorate pollen viability.

Taxon
datee0
Organ / stage
pollene0
Treatment
ultra-low temperature (-196˚C)e0
Outcome
pollen viabilitye0
Direction
without any deteriorating effecte0

Not reportedComparator

Verbatim

e0

“Long-term storage of date pollen using an ultra-low temperature (-196˚C) can be used without any deteriorating effect on pollen viability”

✓ located verbatim in the source · abstract, characters 1125–1262 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Room-temperature storage significantly reduced pollen germination.

Taxon
Date PalmPhoenix dactyliferat0
Organ / stage
pollene0
Treatment
room temperature storagee0
Outcome
pollen germinatione0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“room temperature storage significantly reduced pollen germination”

✓ located verbatim in the source · abstract, characters 1270–1335 · show in text ↑
t0

“Date Palm (Phoenix dactylifera) Pollen”

✓ located verbatim in the source · title, characters 47–85 · show in text ↑

calcium nitrate · growth · Phoenix dactylifera (date palm)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

boric acid · growth · Phoenix dactylifera (date palm)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

sucrose · growth · Phoenix dactylifera (date palm)Results agree

20 papers: 20 positive, 0 negative or no effect

19 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

storage · yield · Phoenix dactylifera (date palm)Results agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.