Abstract
Iran is one of the main date (Phoenix dactylifera) producing countries in the world. however, little information is available on the pollen viability and favorable storage conditions of Iranian male date cultivars. This study was conducted to estimate the best in vitro pollen germination of three male date palm cultivars, `Ghanami’, `Samsmavi ’ and `Gheibani ’ using various doses of boric acid (0, 50, 100 and 200 mg L-1), calcium nitrate (0, 200 and 300 mg L-1) and sucrose (15 % and 25 % (w/v)) in a Modified Brebaker and Kwack (MBK) medium. The results showed that the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose. Once the optimal in vitro germination medium had been established, pollen viability ( % germination) of these three cultivars stored for up to 200 days at room temperature (dark and light), in a refrigerator (4˚C), freezer (-20˚C) and in liquid nitrogen (-196˚C) was compared. For all the tested cultivars, germination declined as storage temperature increased and, by 200 days, the highest survival rate was obtained with cryostored pollen. Long-term storage of date pollen using an ultra-low temperature (-196˚C) can be used without any deteriorating effect on pollen viability, while room temperature storage significantly reduced pollen germination.
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Claims 7
Claim · model-written summary
Best in vitro pollen germination occurred with 50 mg L-1 boric acid.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Organ / stage
- pollent0
- Treatment
- 50 mg L-1 boric acide0
- Outcome
- germinatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”
t0“Date Palm (Phoenix dactylifera) Pollen”
Claim · model-written summary
Best in vitro pollen germination occurred with 200 mg L-1 calcium nitrate.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Organ / stage
- pollent0
- Treatment
- 200 mg L-1 calcium nitratee0
- Outcome
- germinatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”
t0“Date Palm (Phoenix dactylifera) Pollen”
Claim · model-written summary
Best in vitro pollen germination occurred with 15 % (w/v) sucrose.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Organ / stage
- pollent0
- Treatment
- 15 % (w/v) sucrosee0
- Outcome
- germinatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the best germination was achieved with 50 mg L-1 boric acid, 200 mg L-1 calcium nitrate and 15 % (w/v) sucrose”
t0“Date Palm (Phoenix dactylifera) Pollen”
Claim · model-written summary
Pollen germination declined as storage temperature increased across all tested cultivars.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Material
- all the tested cultivarse0
- Organ / stage
- Pollent0
- Treatment
- storage temperaturee0
- Outcome
- germinatione0
- Direction
- declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“For all the tested cultivars, germination declined as storage temperature increased”
t0“Date Palm (Phoenix dactylifera) Pollen”
Claim · model-written summary
Cryostored pollen had the highest survival by 200 days.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Organ / stage
- pollene0
- Treatment
- cryostorede0
- Outcome
- survival ratee0
- Direction
- higheste0
- Qualifier
- by 200 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“by 200 days, the highest survival rate was obtained with cryostored pollen”
t0“Date Palm (Phoenix dactylifera) Pollen”
Claim · model-written summary
Long-term storage at -196˚C did not deteriorate pollen viability.
- Taxon
- datee0
- Organ / stage
- pollene0
- Treatment
- ultra-low temperature (-196˚C)e0
- Outcome
- pollen viabilitye0
- Direction
- without any deteriorating effecte0
Not reportedComparator
Verbatim
e0“Long-term storage of date pollen using an ultra-low temperature (-196˚C) can be used without any deteriorating effect on pollen viability”
Claim · model-written summary
Room-temperature storage significantly reduced pollen germination.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Organ / stage
- pollene0
- Treatment
- room temperature storagee0
- Outcome
- pollen germinatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“room temperature storage significantly reduced pollen germination”
t0“Date Palm (Phoenix dactylifera) Pollen”
The same questions in other papers
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2 papers in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.