Abstract
The subunits of the 11 S storage protein from soybean cultivar CX635-1-1-1 were purified and characterized. Six polypeptides with acidic isoelectric points and four with basic isoelectric points were isolated from the purified storage protein. The acidic polypeptides had phenylalanine, leucine, isoleucine, and arginine at the NH2 termini, while the basic polypeptides all had glycine at the NH2 termini. Amino acid analysis indicated that certain acidic and basic polypeptides contained 3 to 6 times more methionine than the other polypeptides. Since the low nutritional quality of legume storage proteins is due to a deficiency in methionine, this observation will have significance in efforts to improve soybean quality. The purified polypeptides were further characterized by NH2-terminal sequence analysis. Considerable homology was found between the members of individual families of acidic and basic polypeptides, indicating that the members of each family arose from a common ancestral gene. This study showed that the glycinin polypeptide composition is more complex than previous reports indicated, and for the first time characterized the various polypeptides of the 11 S storage protein by structural analysis.
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Claims 8
Claim · model-written summary
Six acidic and four basic polypeptides were isolated.
- Taxon
- soybean
Glycine maxe1 - Material
- CX635-1-1-1e1
- Outcome
- polypeptides with acidic isoelectric pointse0
- Value
- Sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six polypeptides with acidic isoelectric points and four with basic isoelectric points were isolated from the purified storage protein”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Four basic polypeptides were isolated.
- Taxon
- soybeanGlycine maxe1
- Outcome
- basic isoelectric pointse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four with basic isoelectric points were isolated from the purified storage protein”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Acidic polypeptides had four listed amino acids at NH2 termini.
- Taxon
- soybeanGlycine maxe1
- Outcome
- phenylalanine, leucine, isoleucine, and arginine at the NH2 terminie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The acidic polypeptides had phenylalanine, leucine, isoleucine, and arginine at the NH2 termini”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Basic polypeptides had glycine at NH2 termini.
- Taxon
- soybeanGlycine maxe1
- Outcome
- glycine at the NH2 terminie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the basic polypeptides all had glycine at the NH2 termini”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Some polypeptides contained 3 to 6 times more methionine than others.
- Taxon
- soybeanGlycine maxe1
- Outcome
- methioninee0
- Direction
- moree0
- Value
- 3 to 6 timese0
- Comparator
- other polypeptidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“certain acidic and basic polypeptides contained 3 to 6 times more methionine than the other polypeptides”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Sequence analysis found considerable homology within acidic and basic polypeptide families.
- Taxon
- soybeanGlycine maxe1
- Outcome
- Considerable homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Considerable homology was found between the members of individual families of acidic and basic polypeptides”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Authors inferred members of each family arose from a common ancestral gene.
- Taxon
- soybeanGlycine maxe1
- Gene
- common ancestral genee0
- Outcome
- members of each family arosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“members of each family arose from a common ancestral gene”
e1“soybean cultivar CX635-1-1-1”
Claim · model-written summary
Glycinin polypeptide composition was more complex than previously reported.
- Taxon
- soybeanGlycine maxe1
- Outcome
- glycinin polypeptide compositione0
- Direction
- more complexe0
- Comparator
- previous reportse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glycinin polypeptide composition is more complex than previous reports indicated”
e1“soybean cultivar CX635-1-1-1”