Abstract
Abstract. This study was carried out to determine suitable in vitro culture conditions of new ever-bearing strawberry ( Fragaria ananassa Duch.), ‘Goha’. Four-week old plantlets which were derived from the meristem culture were used in this study. Three different culture media including MS, Gamborg B5 and White medium were used for the plant culture and the medium concentrations were at the 5 levels of 1/3×, 1/2×, 1×, 2× and 3×. Sucrose content ranged at four levels of 1, 3, 5 and 8% (w/v). Crown diameter on the MS medium was thicker (2.1 mm) than in other media. Fresh weight on the MS medium was 482 mg, which was heavier than 88 mg or 260 mg of Gamborg B5 or White medium, respectively. Particularly, 1/2MS medium was found to have higher growth rate than these in other treatments (fresh weight, dry weight and D/F rate). Shoot length in the treatment of 1% sucrose concentration was 3.6 cm which was the longest. Shoot length was in inverse proportion to the increasing concentration of sucrose. Fresh weight was increased up to 3% sucrose concentration, but decreased above 5% sucrose concentration. From the results, we found that the best condition for in vitro culture of new ever-bearing strawberry ‘Goha’ was 1/2MS medium supplemented with 1% sucrose concentration.
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Claims 8
Claim · model-written summary
MS medium produced thicker crowns, 2.1 mm, than other media.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- MS mediume0
- Outcome
- Crown diametere0
- Direction
- thickere0
- Value
- 2.1 mme0
- Comparator
- other mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Crown diameter on the MS medium was thicker (2.1 mm) than in other media”
t0“strawberry”
Claim · model-written summary
MS medium produced heavier fresh weight, 482 mg, than Gamborg B5 at 88 mg and White medium at 260 mg.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- MS mediume0
- Outcome
- Fresh weighte0
- Direction
- heaviere0
- Value
- 482 mge0
- Qualifier
- 88 mg or 260 mge0
- Comparator
- Gamborg B5 or White mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fresh weight on the MS medium was 482 mg, which was heavier than 88 mg or 260 mg of Gamborg B5 or White medium, respectively”
t0“strawberry”
Claim · model-written summary
Half-strength MS yielded higher growth rates for fresh weight, dry weight and D/F rate.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 1/2MS mediume0
- Outcome
- fresh weight, dry weight and D/F ratee0
- Direction
- highere0
- Comparator
- other treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1/2MS medium was found to have higher growth rate than these in other treatments (fresh weight, dry weight and D/F rate)”
t0“strawberry”
Claim · model-written summary
One percent sucrose gave the longest shoots, 3.6 cm.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 1% sucrose concentratione0
- Outcome
- Shoot lengthe0
- Direction
- longeste0
- Value
- 3.6 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot length in the treatment of 1% sucrose concentration was 3.6 cm which was the longest”
t0“strawberry”
Claim · model-written summary
Shoot length declined as sucrose concentration rose.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- sucrosee0
- Outcome
- Shoot lengthe0
- Direction
- inverse proportione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot length was in inverse proportion to the increasing concentration of sucrose”
t0“strawberry”
Claim · model-written summary
Fresh weight increased up to 3% sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 3% sucrose concentratione0
- Outcome
- Fresh weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fresh weight was increased up to 3% sucrose concentration”
t0“strawberry”
Claim · model-written summary
Fresh weight declined above 5% sucrose.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- 5% sucrose concentratione0
- Outcome
- Fresh weighte0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fresh weight was increased up to 3% sucrose concentration, but decreased above 5% sucrose concentration”
t0“strawberry”
Claim · model-written summary
The authors identified half-strength MS with 1% sucrose as the best culture condition.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- 1/2MS medium supplemented with 1% sucrose concentratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best condition for in vitro culture of new ever-bearing strawberry ‘Goha’ was 1/2MS medium supplemented with 1% sucrose concentration”
t0“strawberry”
The same questions in other papers
sucrose · growth · Fragaria x ananassa (strawberry)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
8 papers more in this group
See all 12 papers →in vitro · growth · Fragaria x ananassa (strawberry)Positive and negative results
75 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
82 papers more in this group
See all 86 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.