Abstract
The study comprised of experiments for shoot regeneration and plantlet formation from cotyledonary node of Vigna mungo by culturing them on low concentration of BAP followed by transfer to hormone free MS medium. Cotyledonary node explants were cultured on different concentrations of BAP (0, 1, 2.5 and 50 mg l-1). Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium. However, culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant) compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants). The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100.0%) of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA. The rooted plants were transferred to pots for hardening.
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Claims 3
Claim · model-written summary
Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP
- Taxon
- Vigna mungo
Vigna mungot0 - Organ / stage
- cotyledonary nodese1
- Outcome
- Shoot regeneratione1
- Qualifier
- irrespective of the presence or absence of BAPe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of Blackgram (Vigna mungo L.)”
e1“Shoot regeneration occurred from cotyledonary nodes irrespective of the presence or absence of BAP in the medium”
Claim · model-written summary
Culture on 1.0 mg l-1 BAP for 10 days followed by hormone-free medium gave more shoots per explant than hormone-free culture for 15 days followed by 1.0 mg l-1 BAP
- Taxon
- Vigna mungoVigna mungot0
- Treatment
- 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free mediume2
- Outcome
- number of shootse2
- Direction
- highere2
- Value
- 9.33/explante2
- Comparator
- hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAPe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of Blackgram (Vigna mungo L.)”
e2“culture of cotyledonary node explants for 10 days on medium containing 1.0 mg l-1 BAP followed by transfer to hormone free medium gave higher number of shoots (9.33/explant) compared to culture of the explants on hormone free medium for 15 days followed by transfer to medium containing 1.0 mg l-1 BAP (8.33/explants)”
Claim · model-written summary
Rooting frequency of 100% was observed on MS medium supplemented with 0.5 mg l-1 IBA
- Taxon
- Vigna mungoVigna mungot0
- Treatment
- MS medium supplemented with 0.5 mg l-1 IBAe3
- Outcome
- rootinge3
- Value
- 100.0%e3
- Qualifier
- high frequencye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of Blackgram (Vigna mungo L.)”
e3“The high frequency (100.0%) of rooting was observed with MS medium supplemented with 0.5 mg l-1 IBA”
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