Abstract
Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1 was cultured on MS medium containing various concentrations of cytokinins BAP/Kn/TDZ (0.5-3.0 mg/L) alone and also in combination with auxins IAA (0.5 mg/L). Maximum number of shoot bud proliferation was observed at (0.5 mg/L) IAA + (2.0 mg/L).TDZ, compared to all other concentrations of Kn/BAP alone. As the concentration was increased above (2.0mg/L) the shoot bud induction was reduced gradually in both the cytokinins tested. The synergistic effect of both auxin and cytokinin combination was found to be effective in inducing maximum number of shoots. High frequency of shoots was induced at (0.5mg/L IAA + (2.5mg/L) Kn and (0.5mg/L) IAA+ (2.0mg/L) TDZ. The in vitro regenerated shoots produced more number of roots on MS medium containing (1.0mg/L) IBA. Thus the plant developed in vitro using nodal cultures were established in pots containing garden soil outside under shade in wound temperature and light conditions. These plants flowered after 8 weeks following transfer to pots. The protocol established can be used for rapid multiplication of the specific producing true to type plants.
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Claims 8
Claim · model-written summary
IAA plus TDZ produced maximum shoot bud proliferation relative to Kn or BAP alone.
- Taxon
- Black gram
Vigna mungoe1 - Material
- Vamban-1e1
- Treatment
- (0.5 mg/L) IAA + (2.0 mg/L).TDZe0
- Outcome
- number of shoot bud proliferatione0
- Direction
- Maximume0
- Comparator
- Kn/BAP alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum number of shoot bud proliferation was observed at (0.5 mg/L) IAA + (2.0 mg/L).TDZ, compared to all other concentrations of Kn/BAP alone.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
Increasing concentration above 2.0 mg/L gradually reduced shoot bud induction.
- Taxon
- Black gramVigna mungoe1
- Treatment
- concentration was increased above (2.0mg/L)e0
- Outcome
- shoot bud inductione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As the concentration was increased above (2.0mg/L) the shoot bud induction was reduced gradually in both the cytokinins tested.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
The auxin and cytokinin combination induced the maximum number of shoots.
- Taxon
- Black gramVigna mungoe1
- Treatment
- auxin and cytokinin combinatione0
- Outcome
- number of shootse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The synergistic effect of both auxin and cytokinin combination was found to be effective in inducing maximum number of shoots.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
IAA plus Kn induced a high frequency of shoots.
- Taxon
- Black gramVigna mungoe1
- Treatment
- (0.5mg/L IAA + (2.5mg/L) Kne0
- Outcome
- frequency of shootse0
- Direction
- Highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency of shoots was induced at (0.5mg/L IAA + (2.5mg/L) Kn”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
Regenerated shoots produced more roots on MS medium with IBA.
- Taxon
- Black gramVigna mungoe1
- Treatment
- MS medium containing (1.0mg/L) IBAe0
- Outcome
- number of rootse0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro regenerated shoots produced more number of roots on MS medium containing (1.0mg/L) IBA.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
Regenerated plants established in pots with garden soil.
- Taxon
- Black gramVigna mungoe1
- Outcome
- established in potse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plant developed in vitro using nodal cultures were established in pots containing garden soil outside under shade”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
Regenerated plants flowered eight weeks after transfer to pots.
- Taxon
- Black gramVigna mungoe1
- Outcome
- flowerede0
- Value
- 8 weekse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants flowered after 8 weeks following transfer to pots.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
Claim · model-written summary
The protocol was proposed for rapid multiplication of true-to-type plants.
- Taxon
- Black gramVigna mungoe1
- Outcome
- rapid multiplication of the specific producing true to type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protocol established can be used for rapid multiplication of the specific producing true to type plants.”
e1“Nodal explants of Black gram (Vigna mungo L.) Hepper Var Vamban-1”
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