Abstract
Changes in glycinin-digesting protease activity during soybean germination have been investigated. The glycinin-digesting protease activities of imbibed or germinated soybean seed were assayed by RP‒HPLC using a tryptic peptide from CM‒glycinin or by SDS‒PAGE using CM‒glycinin as the endogenous substrate. Proteolytic activities of the germinated soybean seeds were found through the whole period of germination, the activities were maintained significantly unchanged during germination for 4 days, and then those specific activities declined slowly. AE‒HPLC analysis of the glycinin-digesting protease in the imbibed or germinated soybean seeds showed unchanged peaks corresponding to glycinin- digesting activity, suggesting that the glycinin-digesting protease was not induced during germination but had already been synthesized during seed maturation.
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Claims 5
Claim · model-written summary
Germinated soybean seeds showed proteolytic activity throughout germination.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- germinated soybean seedse0
- Gene
- glycinin-digesting proteaset0
- Outcome
- Proteolytic activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proteolytic activities of the germinated soybean seeds were found through the whole period of germination”
t0“glycinin-digesting protease activity during soybean germination”
Claim · model-written summary
Soybean seed proteolytic activity remained significantly unchanged for four days of germination.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- activitiese0
- Direction
- unchangede0
- Value
- 4 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activities were maintained significantly unchanged during germination for 4 days”
t0“During Soybean Germination.”
Claim · model-written summary
Specific proteolytic activities then slowly declined.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- specific activitiese0
- Direction
- declinede0
- Qualifier
- slowlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“then those specific activities declined slowly”
t0“During Soybean Germination.”
Claim · model-written summary
AE-HPLC peaks corresponding to glycinin-digesting activity were unchanged during germination.
- Taxon
- soybeanGlycine maxe0
- Gene
- glycinin-digesting proteasee0
- Outcome
- peaks corresponding to glycinin- digesting activitye0
- Direction
- unchangede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AE‒HPLC analysis of the glycinin-digesting protease in the imbibed or germinated soybean seeds showed unchanged peaks corresponding to glycinin- digesting activity”
Claim · model-written summary
The protease was inferred to have been synthesized during seed maturation rather than induced during germination.
- Taxon
- SoybeanGlycine maxt0
- Gene
- glycinin-digesting proteasee0
- Outcome
- not induced during germination but had already been synthesized during seed maturatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that the glycinin-digesting protease was not induced during germination but had already been synthesized during seed maturation”
t0“During Soybean Germination.”