Abstract
S>A ten-fold increase in asparagine synthetase (EC 6.3.1.1) activity was obtained in the bud tissue within 5 hr after 10 krad gamma irradiation. This was followed by a rapid fall in this activity. Irradiated buds also showed an increased incorporation of /sup 14/Cleucine into their soluble protein during this period. Cycloheximide (5 mu g/ml) and puromycin (25 mu g/ml) inhibited this protein synthesis as well as enzyme activation to about 70 and 60%, respectively. Actinomycin D (10 mu g/ml) inhibited protein synthesis in irradiated buds to 70%, while no inhibition was observed with unirradiated buds. The enhancement of asparagine synthetase activity was also arrested to about 70% by actinomycin D. Purified asparagine synthetase showed a single band on polyacrylamide gel electrophoresis. A good correlation was obtained between the incorporation of /sup 14/C --leucine into the enzyme fraction and increase in specific activity. (auth)
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Claims 10
Claim · model-written summary
Gamma irradiation increased bud asparagine synthetase activity tenfold within five hours.
- Taxon
- potatoes
Solanum tuberosumt0 - Organ / stage
- bud tissuee0
- Treatment
- 10 krad gamma irradiatione0
- Gene
- asparagine synthetase (EC 6.3.1.1)e0
- Outcome
- asparagine synthetase (EC 6.3.1.1) activitye0
- Direction
- increasee0
- Value
- ten-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“A ten-fold increase in asparagine synthetase (EC 6.3.1.1) activity was obtained in the bud tissue within 5 hr after 10 krad gamma irradiation”
Claim · model-written summary
Enzyme activity then fell rapidly.
- Taxon
- potatoesSolanum tuberosumt0
- Outcome
- this activitye0
- Direction
- falle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“This was followed by a rapid fall in this activity”
Claim · model-written summary
Irradiated buds had increased leucine incorporation into soluble protein.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Irradiatede0
- Outcome
- incorporation of /sup 14/Cleucine into their soluble proteine0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Irradiated buds also showed an increased incorporation of /sup 14/Cleucine into their soluble protein during this period”
Claim · model-written summary
Cycloheximide inhibited protein synthesis and enzyme activation by about 70%.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Cycloheximide (5 mu g/ml)e0
- Outcome
- protein synthesis as well as enzyme activatione0
- Direction
- inhibitede0
- Value
- 70e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Cycloheximide (5 mu g/ml) and puromycin (25 mu g/ml) inhibited this protein synthesis as well as enzyme activation to about 70 and 60%, respectively”
Claim · model-written summary
Puromycin inhibited protein synthesis and enzyme activation by about 60%.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- puromycin (25 mu g/ml)e0
- Outcome
- protein synthesis as well as enzyme activatione0
- Direction
- inhibitede0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Cycloheximide (5 mu g/ml) and puromycin (25 mu g/ml) inhibited this protein synthesis as well as enzyme activation to about 70 and 60%, respectively”
Claim · model-written summary
Actinomycin D inhibited protein synthesis in irradiated buds to 70%.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- Actinomycin D (10 mu g/ml)e0
- Outcome
- protein synthesis in irradiated budse0
- Direction
- inhibitede0
- Value
- 70%e0
- Comparator
- unirradiated budse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Actinomycin D (10 mu g/ml) inhibited protein synthesis in irradiated buds to 70%, while no inhibition was observed with unirradiated buds”
Claim · model-written summary
Actinomycin D did not inhibit protein synthesis in unirradiated buds.
- Taxon
- potatoesSolanum tuberosumt0
- Outcome
- inhibitione0
- Direction
- no inhibitione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“no inhibition was observed with unirradiated buds”
Claim · model-written summary
Actinomycin D arrested enhanced asparagine synthetase activity to about 70%.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- actinomycin De0
- Outcome
- asparagine synthetase activitye0
- Direction
- arrestede0
- Value
- 70%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“The enhancement of asparagine synthetase activity was also arrested to about 70% by actinomycin D”
Claim · model-written summary
Purified asparagine synthetase formed one electrophoresis band.
- Taxon
- potatoesSolanum tuberosumt0
- Gene
- asparagine synthetasee0
- Outcome
- single band on polyacrylamide gel electrophoresise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Purified asparagine synthetase showed a single band on polyacrylamide gel electrophoresis”
Claim · model-written summary
Leucine incorporation into the enzyme fraction correlated with increased specific activity.
- Taxon
- potatoesSolanum tuberosumt0
- Compound
- /sup 14/C --leucinee0
- Outcome
- specific activitye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“A good correlation was obtained between the incorporation of /sup 14/C --leucine into the enzyme fraction and increase in specific activity”
The same questions in other papers
radiation · physiology: osmolytes · Solanum tuberosum (potato)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.