Abstract
Plant endo-β-1,4-glucanases (EGases) include cell wall-modifying enzymes that are involved in nematode-induced growth of syncytia (feeding structures) in nematode-infected roots. EGases in the α- and β-subfamilies contain signal peptides and are secreted, whereas those in the γ-subfamily have a membrane-anchoring domain and are not secreted. The Arabidopsis α-EGase At1g48930, designated as AtCel6, is known to be down-regulated by beet cyst nematode (Heterodera schachtii) in Arabidopsis roots, whereas another α-EGase, AtCel2, is up-regulated. Here, we report that the ectopic expression of AtCel6 in soybean roots reduces susceptibility to both soybean cyst nematode (SCN; Heterodera glycines) and root knot nematode (Meloidogyne incognita). Suppression of GmCel7, the soybean homologue of AtCel2, in soybean roots also reduces the susceptibility to SCN. In contrast, in studies on two γ-EGases, both ectopic expression of AtKOR2 in soybean roots and suppression of the soybean homologue of AtKOR3 had no significant effect on SCN parasitism. Our results suggest that secreted α-EGases are likely to be more useful than membrane-bound γ-EGases in the development of an SCN-resistant soybean through gene manipulation. Furthermore, this study provides evidence that Arabidopsis shares molecular events of cyst nematode parasitism with soybean, and confirms the suitability of the Arabidopsis-H. schachtii interaction as a model for the soybean-H. glycines pathosystem.
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Claims 8
Claim · model-written summary
Ectopic AtCel6 expression reduced soybean root susceptibility to soybean cyst nematode.
- Taxon
- soybean
Glycine maxe0 - Treatment
- ectopic expression of AtCel6e0
- Interactor
- Heterodera glycines
Heterodera glycinese0pathogen - Gene
- AtCel6e0
- Outcome
- susceptibility to both soybean cyst nematodee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of AtCel6 in soybean roots reduces susceptibility to both soybean cyst nematode (SCN; Heterodera glycines) and root knot nematode (Meloidogyne incognita)”
Claim · model-written summary
Ectopic AtCel6 expression reduced soybean root susceptibility to root knot nematode.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ectopic expression of AtCel6e0
- Interactor
- Meloidogyne incognita
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogyne incognitae0pathogen - Gene
- AtCel6e0
- Outcome
- susceptibility to both soybean cyst nematode (SCN; Heterodera glycines) and root knot nematodee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“ectopic expression of AtCel6 in soybean roots reduces susceptibility to both soybean cyst nematode (SCN; Heterodera glycines) and root knot nematode (Meloidogyne incognita)”
Claim · model-written summary
Suppressing GmCel7 reduced soybean susceptibility to SCN.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Suppression of GmCel7e0
- Interactor
- SCNe0pathogen
- Gene
- GmCel7e0
- Outcome
- susceptibility to SCNe0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of GmCel7, the soybean homologue of AtCel2, in soybean roots also reduces the susceptibility to SCN”
Claim · model-written summary
Ectopic AtKOR2 expression had no significant effect on SCN parasitism.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ectopic expression of AtKOR2e0
- Interactor
- SCNe0pathogen
- Gene
- AtKOR2e0
- Outcome
- SCN parasitisme0
- Direction
- no significant effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both ectopic expression of AtKOR2 in soybean roots and suppression of the soybean homologue of AtKOR3 had no significant effect on SCN parasitism”
Claim · model-written summary
Suppressing the soybean homologue of AtKOR3 had no significant effect on SCN parasitism.
- Taxon
- soybeanGlycine maxe0
- Treatment
- suppression of the soybean homologue of AtKOR3e0
- Interactor
- SCNe0pathogen
- Gene
- AtKOR3e0
- Outcome
- SCN parasitisme0
- Direction
- no significant effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of the soybean homologue of AtKOR3 had no significant effect on SCN parasitism”
Claim · model-written summary
The authors suggest secreted α-EGases are more useful than membrane-bound γ-EGases for SCN-resistant soybean development.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SCNe0pathogen
- Gene
- α-EGasese0
- Outcome
- more useful than membrane-bound γ-EGases in the development of an SCN-resistant soybeane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“secreted α-EGases are likely to be more useful than membrane-bound γ-EGases in the development of an SCN-resistant soybean through gene manipulation”
Claim · model-written summary
The study supports shared cyst nematode parasitism events in Arabidopsis and soybean.
- Taxon
- Arabidopsis
Arabidopsise0 - Outcome
- molecular events of cyst nematode parasitisme0
- Comparator
- soybeane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis shares molecular events of cyst nematode parasitism with soybean”
Claim · model-written summary
The Arabidopsis–H. schachtii interaction is supported as a model for the soybean–H. glycines pathosystem.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- H. schachtiiHeterodera schachtiie0pathogen
- Outcome
- suitability of the Arabidopsis-H. schachtii interaction as a modele0
- Comparator
- soybean-H. glycines pathosysteme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confirms the suitability of the Arabidopsis-H. schachtii interaction as a model for the soybean-H. glycines pathosystem”
The same questions in other papers
overexpression · Heterodera glycines · Glycine max (soybean)Positive and negative results
1 in the opposite direction
- negative
loss of function · disease · Glycine max (soybean)Positive and negative results
16 papers in the same direction as this paper · 20 in the opposite direction
- negative
- negative
- negative
33 papers more in this group
See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.