Abstract
Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean GmPUB genes to soybean cyst nematode (SCN, Heterodera glycines) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 GmPUB genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that GmPUB genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24A were dramatically changed during SCN infection. Furthermore, functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress. The results from our present study provide insights into the complicated molecular mechanism of the interaction between soybean and SCN.
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Claims 5
Claim · model-written summary
GmPUB genes responded to cyst nematode infection at four post-inoculation times.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- cyst nematodee0pathogen
- Gene
- GmPUB genese0
- Outcome
- responded to cyst nematode infectione0
- Qualifier
- 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPUB genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi”
t0“Soybean”
Claim · model-written summary
Expression of six named GmPUB genes changed dramatically during infection.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Heterodera glycines
Heterodera glycinese1pathogen - Gene
- GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24Ae0
- Outcome
- expression levelse0
- Direction
- dramatically changede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels of GmPUB16A, GmPUB20A, GmCHIPA, GmPUB33A, GmPUB23A and GmPUB24A were dramatically changed during SCN infection”
t0“Soybean”
e1“soybean cyst nematode (SCN, Heterodera glycines) infection”
Claim · model-written summary
The gene GmPUB20A negatively regulated soybean resistance to SCN.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- overexpression and RNAie0
- Interactor
- Heterodera glycinesHeterodera glycinese1pathogen
- Gene
- GmPUB20Ae0
- Outcome
- soybean resistancee0
- Direction
- negatively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress”
t0“Soybean”
e1“soybean cyst nematode (SCN, Heterodera glycines) infection”
Claim · model-written summary
The gene GmPUB33A negatively regulated soybean resistance to SCN.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- overexpression and RNAie0
- Interactor
- Heterodera glycinesHeterodera glycinese1pathogen
- Gene
- GmPUB33Ae0
- Outcome
- soybean resistancee0
- Direction
- negatively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress”
t0“Soybean”
e1“soybean cyst nematode (SCN, Heterodera glycines) infection”
Claim · model-written summary
The gene GmPUB24A negatively regulated soybean resistance to SCN.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- overexpression and RNAie0
- Interactor
- Heterodera glycinesHeterodera glycinese1pathogen
- Gene
- GmPUB24Ae0
- Outcome
- soybean resistancee0
- Direction
- negatively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional analysis of these GmPUB genes by overexpression and RNAi showed that GmPUB20A, GmPUB33A and GmPUB24A negatively regulated soybean resistance under SCN stress”
t0“Soybean”
e1“soybean cyst nematode (SCN, Heterodera glycines) infection”
The same questions in other papers
overexpression · Heterodera glycines · Glycine max (soybean)Positive and negative results
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- positive
loss of function · Heterodera glycines · Glycine max (soybean)Positive and negative results
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- negative
- positive
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.