Abstract
Soybean cyst nematode (SCN, Heterodera glycines) is an obligate sedentary biotroph that poses major threats to soybean production globally. Recently, multiple miRNAome studies revealed that miRNAs participate in complicated soybean-SCN interactions by regulating their target genes. However, the functional roles of miRNA and target genes regulatory network are still poorly understood. In present study, we firstly investigated the expression patterns of miR159 and targeted GmMYB33 genes. The results showed miR159-3p downregulation during SCN infection; conversely, GmMYB33 genes upregulated. Furthermore, miR159 overexpressing and silencing soybean hairy roots exhibited strong resistance and susceptibility to H. glycines, respectively. In particular, miR159-GAMYB genes are reported to be involve in GA signaling and metabolism. Therefore, we then investigated the effects of GA application on the expression of miR159-GAMYB module and the development of H. glycines. We found that GA directly controls the miR159-GAMYB module, and exogenous GA application enhanced endogenous biologically active GA1 and GA3, the abundance of miR159, lowered the expression of GmMYB33 genes and delayed the development of H. glycines. Moreover, SCN infection also results in endogenous GA content decreased in soybean roots. In summary, the soybean miR159-GmMYB33 module was directly involved in the GA-modulated soybean resistance to H. glycines.
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Claims 9
Claim · model-written summary
miR159-3p decreased during nematode infection.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- SCNe0pathogen
- Gene
- miR159-3pe0
- Outcome
- miR159-3p downregulatione0
- Direction
- downregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR159-3p downregulation during SCN infection”
t0“Soybean”
Claim · model-written summary
GmMYB33 genes increased during nematode infection.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- Heterodera glycines
Heterodera glycinest0pathogen - Gene
- GmMYB33 genese0
- Outcome
- GmMYB33 genes upregulatede0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“conversely, GmMYB33 genes upregulated”
t0“Soybean miR159-GmMYB33 Regulatory Network Involved in Gibberellin-Modulated Resistance to Heterodera glycines”
Claim · model-written summary
miR159 overexpression produced strong nematode resistance.
- Taxon
- soybeanGlycine maxe0
- Treatment
- miR159 overexpressinge0
- Interactor
- H. glycinesHeterodera glycinese0pathogen
- Gene
- miR159e0
- Outcome
- strong resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR159 overexpressing and silencing soybean hairy roots exhibited strong resistance and susceptibility to H. glycines, respectively”
Claim · model-written summary
miR159 silencing produced nematode susceptibility.
- Taxon
- soybeanGlycine maxe0
- Treatment
- silencinge0
- Interactor
- H. glycinesHeterodera glycinese0pathogen
- Gene
- miR159e0
- Outcome
- susceptibilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR159 overexpressing and silencing soybean hairy roots exhibited strong resistance and susceptibility to H. glycines, respectively”
Claim · model-written summary
GA application raised active GA1 and GA3 in soybean.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- exogenous GA applicatione0
- Outcome
- endogenous biologically active GA1 and GA3e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA application enhanced endogenous biologically active GA1 and GA3”
t0“Soybean”
Claim · model-written summary
GA application raised miR159 abundance.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- exogenous GA applicatione0
- Gene
- miR159e0
- Outcome
- abundance of miR159e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA application enhanced endogenous biologically active GA1 and GA3, the abundance of miR159”
t0“Soybean”
Claim · model-written summary
GA application lowered GmMYB33 expression.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- exogenous GA applicatione0
- Gene
- GmMYB33 genese0
- Outcome
- expression of GmMYB33 genese0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA application enhanced endogenous biologically active GA1 and GA3, the abundance of miR159, lowered the expression of GmMYB33 genes”
t0“Soybean”
Claim · model-written summary
GA application delayed nematode development.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- exogenous GA applicatione0
- Interactor
- H. glycinesHeterodera glycinese0pathogen
- Outcome
- development of H. glycinese0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA application enhanced endogenous biologically active GA1 and GA3, the abundance of miR159, lowered the expression of GmMYB33 genes and delayed the development of H. glycines”
t0“Soybean”
Claim · model-written summary
Nematode infection reduced endogenous GA in soybean roots.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- rootse0
- Interactor
- SCNe0pathogen
- Outcome
- endogenous GA contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SCN infection also results in endogenous GA content decreased in soybean roots”
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.