Abstract
The somatic embryogenesis is an advanced method for clonal propagation and a useful tool for ex situ conservation of genetic resources. In this paper an experimental device was designed, composed of: one oak species (Quercus petraea), one provenance, four types of explants (developmental stages of the zygotic embryo) and four variants of culture medium. It was released the two-way statistical analyses of the two analyzed factors affecting the embryo induction efficiency: the stage of explant and culture medium (4 stages x 4 culture media). The most important factor affecting the efficiency of somatic embryogenesis was found to be the developmental stage of the zygotic embryos used as juvenile explants. The high embryogenic ability of young zygotic embryos and low embryogenic ability of maturing zygotic ones showed the strong positive relationship between somatic embryo induction and accumulation of reserve nutrients in cotyledons. The best results were obtained with the youngest embryos (stage 1), with a linear decrement towards the stage 4. The embryo induction efficiency ranged between 44% and 10% in Q. petraea. Two nutritive culture media and two growth hormone combinations were tested. The effect of culture medium proved to be totally insignificant, besides the differences observed among the variants cultivated on different media. The situation was the same as well for the nutrients as for growth regulators.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Zygotic embryo developmental stage was the most important factor for somatic embryogenesis efficiency.
- Taxon
- Quercus petraea
Quercus petraeat0 - Organ / stage
- developmental stage of the zygotic embryose0
- Outcome
- efficiency of somatic embryogenesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most important factor affecting the efficiency of somatic embryogenesis was found to be the developmental stage of the zygotic embryos used as juvenile explants.”
t0“Quercus petraea”
Claim · model-written summary
Young zygotic embryos had high embryogenic ability.
- Taxon
- Quercus petraeaQuercus petraeat0
- Organ / stage
- young zygotic embryose0
- Outcome
- high embryogenic abilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high embryogenic ability of young zygotic embryos and low embryogenic ability of maturing zygotic ones”
t0“Quercus petraea”
Claim · model-written summary
Maturing zygotic embryos had low embryogenic ability.
- Taxon
- Quercus petraeaQuercus petraeat0
- Organ / stage
- maturing zygotic onese0
- Outcome
- low embryogenic abilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high embryogenic ability of young zygotic embryos and low embryogenic ability of maturing zygotic ones”
t0“Quercus petraea”
Claim · model-written summary
Embryo induction was best for stage 1 and declined linearly toward stage 4.
- Taxon
- Quercus petraeaQuercus petraeat0
- Organ / stage
- youngest embryos (stage 1)e0
- Outcome
- best resultse0
- Direction
- linear decremente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best results were obtained with the youngest embryos (stage 1), with a linear decrement towards the stage 4.”
t0“Quercus petraea”
Claim · model-written summary
Embryo induction efficiency ranged from 44% to 10%.
- Taxon
- Q. petraeaQuercus petraeae0
- Outcome
- embryo induction efficiencye0
- Value
- 44% and 10%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryo induction efficiency ranged between 44% and 10% in Q. petraea.”
Claim · model-written summary
Culture medium showed no significant effect on embryo induction.
- Taxon
- Quercus petraeaQuercus petraeat0
- Treatment
- culture mediume0
- Outcome
- effect of culture mediume0
- Direction
- totally insignificante0
- Comparator
- different mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of culture medium proved to be totally insignificant, besides the differences observed among the variants cultivated on different media.”
t0“Quercus petraea”
Claim · model-written summary
Neither nutrients nor growth regulators showed a significant medium effect.
- Taxon
- Quercus petraeaQuercus petraeat0
- Outcome
- the nutrients as for growth regulatorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The situation was the same as well for the nutrients as for growth regulators.”
t0“Quercus petraea”