Abstract
The seeds of Arabidopsis thaliana and many other plants are surrounded by a pectinaceous mucilage that aids in seed hydration and germination. Mucilage is synthesized during seed development within maternally derived seed coat mucilage secretory cells (MSCs), and is released to surround the seed upon imbibition. The FEI1/FEI2 receptor-like kinases and the SOS5 extracellular GPI-anchored protein were shown previously to act on a pathway that regulates the synthesis of cellulose in Arabidopsis roots. Here, we demonstrate that both FEI2 and SOS5 also play a role in the synthesis of seed mucilage. Disruption of FEI2 or SOS5 leads to a reduction in the rays of cellulose observed across the seed mucilage inner layer, which alters the structure of the mucilage in response to hydration. Mutations in CESA5, which disrupts an isoform of cellulose synthase involved in primary cell wall synthesis, result in a similar seed mucilage phenotype. The data indicate that CESA5-derived cellulose plays an important role in the synthesis and structure of seed coat mucilage and that the FEI2/SOS5 pathway plays a role in the regulation of cellulose synthesis in MSCs. Moreover, these results establish a novel structural role for cellulose in anchoring the pectic component of seed coat mucilage to the seed surface.
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Claims 6
Claim · model-written summary
Disrupting FEI2 reduces cellulose rays across the seed mucilage inner layer and changes hydrated mucilage structure.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- seed mucilage inner layere0
- Treatment
- Disruption of FEI2e0
- Gene
- FEI2e0
- Outcome
- rays of cellulose observed across the seed mucilage inner layere0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FEI2 or SOS5 leads to a reduction in the rays of cellulose observed across the seed mucilage inner layer, which alters the structure of the mucilage in response to hydration.”
t0“Arabidopsis”
Claim · model-written summary
Disrupting SOS5 reduces cellulose rays across the seed mucilage inner layer and changes hydrated mucilage structure.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Disruption of FEI2 or SOS5e0
- Gene
- SOS5e0
- Outcome
- rays of cellulose observed across the seed mucilage inner layere0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FEI2 or SOS5 leads to a reduction in the rays of cellulose observed across the seed mucilage inner layer, which alters the structure of the mucilage in response to hydration.”
t0“Arabidopsis”
Claim · model-written summary
CESA5 mutations produce a similar seed mucilage phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in CESA5e0
- Gene
- CESA5e0
- Outcome
- similar seed mucilage phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in CESA5, which disrupts an isoform of cellulose synthase involved in primary cell wall synthesis, result in a similar seed mucilage phenotype.”
t0“Arabidopsis”
Claim · model-written summary
CESA5-derived cellulose contributes to seed coat mucilage synthesis and structure.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CESA5e0
- Outcome
- synthesis and structure of seed coat mucilagee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CESA5-derived cellulose plays an important role in the synthesis and structure of seed coat mucilage”
t0“Arabidopsis”
Claim · model-written summary
The FEI2/SOS5 pathway regulates cellulose synthesis in mucilage secretory cells.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- FEI2/SOS5e0
- Outcome
- regulation of cellulose synthesis in MSCse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the FEI2/SOS5 pathway plays a role in the regulation of cellulose synthesis in MSCs”
t0“Arabidopsis”
Claim · model-written summary
Cellulose anchors the pectic mucilage component to the seed surface.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- anchoring the pectic component of seed coat mucilage to the seed surfacee0
- Qualifier
- cellulosee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cellulose in anchoring the pectic component of seed coat mucilage to the seed surface”
t0“Arabidopsis”
The same questions in other papers
loss of function · molecular: metabolites · ArabidopsisPositive and negative results
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- no effect
- negative
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